dc.contributor.author |
Naylor, Richard |
en |
dc.contributor.author |
McGhee, Charles |
en |
dc.contributor.author |
Cowan, Chad A |
en |
dc.contributor.author |
Davidson, Alan |
en |
dc.contributor.author |
Holm, Teresa M |
en |
dc.contributor.author |
Sherwin, Trevor |
en |
dc.date.accessioned |
2018-10-14T22:21:40Z |
en |
dc.date.issued |
2016-01 |
en |
dc.identifier.issn |
1932-6203 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/41350 |
en |
dc.description.abstract |
Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based therapies may offer an alternative approach given that the eye is amenable to such treatments and corneal diseases like keratoconus have been associated specifically with the death of corneal keratocytes. The ability to generate corneal keratocytes in vitro may enable a cell-based therapy to treat patients with keratoconus. Human induced pluripotent stem cells (hiPSCs) offer an abundant supply of cells from which any cell in the body can be derived. In the present study, hiPSCs were successfully differentiated into neural crest cells (NCCs), the embryonic precursor to keratocytes, and then cultured on cadaveric corneal tissue to promote keratocyte differentiation. The hiPSC-derived NCCs were found to migrate into the corneal stroma where they acquired a keratocyte-like morphology and an expression profile similar to corneal keratocytes in vivo. These results indicate that hiPSCs can be used to generate corneal keratocytes in vitro and lay the foundation for using these cells in cornea cell-based therapies. |
en |
dc.format.medium |
Electronic-eCollection |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
PloS one |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1932-6203/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Iris |
en |
dc.subject |
Cell Line |
en |
dc.subject |
Neural Crest |
en |
dc.subject |
Humans |
en |
dc.subject |
Collagen |
en |
dc.subject |
Cell Culture Techniques |
en |
dc.subject |
Cell Differentiation |
en |
dc.subject |
Phenotype |
en |
dc.subject |
Induced Pluripotent Stem Cells |
en |
dc.subject |
Corneal Keratocytes |
en |
dc.title |
Derivation of Corneal Keratocyte-Like Cells from Human Induced Pluripotent Stem Cells. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1371/journal.pone.0165464 |
en |
pubs.issue |
10 |
en |
pubs.begin-page |
e0165464 |
en |
pubs.volume |
11 |
en |
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
27792791 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
544372 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Ophthalmology Department |
en |
dc.identifier.eissn |
1932-6203 |
en |
pubs.record-created-at-source-date |
2016-10-28 |
en |
pubs.dimensions-id |
27792791 |
en |