Human sinoatrial node structure: 3D microanatomy of sinoatrial conduction pathways.

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dc.contributor.author Csepe, Thomas A en
dc.contributor.author Zhao, Jichao en
dc.contributor.author Hansen, Brian J en
dc.contributor.author Li, Ning en
dc.contributor.author Sul, Lidiya V en
dc.contributor.author Lim, Praise en
dc.contributor.author Wang, Yufeng en
dc.contributor.author Simonetti, Orlando P en
dc.contributor.author Kilic, Ahmet en
dc.contributor.author Mohler, Peter J en
dc.contributor.author Janssen, Paul ML en
dc.contributor.author Fedorov, Vadim V en
dc.date.accessioned 2018-10-14T23:08:24Z en
dc.date.issued 2016-01 en
dc.identifier.issn 0079-6107 en
dc.identifier.uri http://hdl.handle.net/2292/41424 en
dc.description.abstract Despite a century of extensive study on the human sinoatrial node (SAN), the structure-to-function features of specialized SAN conduction pathways (SACP) are still unknown and debated. We report a new method for direct analysis of the SAN microstructure in optically-mapped human hearts with and without clinical history of SAN dysfunction.Two explanted donor human hearts were coronary-perfused and optically-mapped. Structural analyses of histological sections parallel to epicardium (∼13-21 μm intervals) were integrated with optical maps to create 3D computational reconstructions of the SAN complex. High-resolution fiber fields were obtained using 3D Eigen-analysis of the structure tensor, and used to analyze SACP microstructure with a fiber-tracking approach.Optical mapping revealed normal SAN activation of the atria through a lateral SACP proximal to the crista terminalis in Heart #1 but persistent SAN exit block in diseased Heart #2. 3D structural analysis displayed a functionally-observed SAN border composed of fibrosis, fat, and/or discontinuous fibers between SAN and atria, which was only crossed by several branching myofiber tracts in SACP regions. Computational 3D fiber-tracking revealed that myofiber tracts of SACPs created continuous connections between SAN #1 and atria, but in SAN #2, SACP region myofiber tracts were discontinuous due to fibrosis and fat.We developed a new integrative functional, structural and computational approach that allowed for the resolution of the specialized 3D microstructure of human SACPs for the first time. Application of this integrated approach will shed new light on the role of the specialized SAN microanatomy in maintaining sinus rhythm. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Progress in biophysics and molecular biology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Heart Conduction System en
dc.subject Sinoatrial Node en
dc.subject Humans en
dc.subject Biological Clocks en
dc.subject Models, Anatomic en
dc.title Human sinoatrial node structure: 3D microanatomy of sinoatrial conduction pathways. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.pbiomolbio.2015.12.011 en
pubs.issue 1-3 en
pubs.begin-page 164 en
pubs.volume 120 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 26743207 en
pubs.end-page 178 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.subtype Research Support, N.I.H., Extramural en
pubs.elements-id 517666 en
pubs.org-id Bioengineering Institute en
pubs.org-id ABI Associates en
dc.identifier.eissn 1873-1732 en
pubs.record-created-at-source-date 2016-03-26 en
pubs.dimensions-id 26743207 en


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