dc.contributor.author |
Mohammadi, M |
en |
dc.contributor.author |
Guan, Jian |
en |
dc.contributor.author |
Khodagholi, F |
en |
dc.contributor.author |
Yans, A |
en |
dc.contributor.author |
Khalaj, S |
en |
dc.contributor.author |
Gholami, M |
en |
dc.contributor.author |
Taghizadeh, GH |
en |
dc.contributor.author |
Aliaghaei, A |
en |
dc.contributor.author |
Abdollahi, M |
en |
dc.contributor.author |
Ghahremani, MH |
en |
dc.contributor.author |
Sharifzadeh, M |
en |
dc.date.accessioned |
2018-10-14T23:31:57Z |
en |
dc.date.issued |
2016-05 |
en |
dc.identifier.issn |
0028-1298 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/41444 |
en |
dc.description.abstract |
Autophagy, the process of self-degradation of cellular components, has an important role in neurodegenerative diseases, such as Alzheimer's disease. In this study, we investigated the effects of SP600125 as c-Jun N-terminal kinase (JNK) inhibitor and bucladesine as a cyclic adenosine 3',5'-monophosphate (cAMP) analog on spatial memory and expression of autophagic factors in Aβ-injected rats. Male Wistar rats were used. Rats were randomly allocated into five groups as following: amyloid beta (Aβ)-only group, Aβ + SP600125 (30 μg/1 μ/side, n = 7) and/or bucladesine (100 μM/1 μl/side, n = 7), and the normal control (vehicle only) group. The treatments were administered bilaterally to the CA1 sub-region of the hippocampus stereotaxically. Spatial reference memory was performed using Morris Water Maze 21 days later. The expression of authophagy markers (beclin1, Atg7, Atg12, and LC3 II/LC3 I) in the hippocampus was evaluated using western blotting. Compared to the vehicle group, Aβ administration reduced spatial reference learning (P < 0.001) and memory (P < 0.01) and upregulated the expression of beclin1, Atg7, Atg12, and LC3 II/I (P < 0.0001). Compare to Aβ-only group, the administration of SP600125 and/or bucladesine improved spatial reference learning (P < 0.001) and memory (P < 0.01). Compared to the Aβ-only group, the treatment with SP600125 and/or bucladesine also reduced beclin1, Atg7, Atg12, and LC3 II/I (P < 0.0001) which was similar to amount of normal rats. In summary, it seems that the improvement of spatial memory by SP600125 and/or bucladesine in Aβ-injected rats is in relation with normalizing of autophagy to the physiologic level, possibly through neuroprotection and/or neuroplasticity. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Naunyn-Schmiedeberg's archives of pharmacology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Animals |
en |
dc.subject |
Rats, Wistar |
en |
dc.subject |
Alzheimer Disease |
en |
dc.subject |
Memory Disorders |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Anthracenes |
en |
dc.subject |
JNK Mitogen-Activated Protein Kinases |
en |
dc.subject |
Microtubule-Associated Proteins |
en |
dc.subject |
Bucladesine |
en |
dc.subject |
Neuroprotective Agents |
en |
dc.subject |
Protein Kinase Inhibitors |
en |
dc.subject |
Drug Therapy, Combination |
en |
dc.subject |
Maze Learning |
en |
dc.subject |
Autophagy |
en |
dc.subject |
Male |
en |
dc.subject |
Amyloid beta-Peptides |
en |
dc.subject |
Spatial Memory |
en |
dc.subject |
Autophagy-Related Protein 7 |
en |
dc.subject |
Autophagy-Related Protein 12 |
en |
dc.subject |
Beclin-1 |
en |
dc.title |
Reduction of autophagy markers mediated protective effects of JNK inhibitor and bucladesine on memory deficit induced by Aβ in rats. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s00210-016-1222-x |
en |
pubs.issue |
5 |
en |
pubs.begin-page |
501 |
en |
pubs.volume |
389 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
26899864 |
en |
pubs.end-page |
510 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
524200 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Pharmacology |
en |
dc.identifier.eissn |
1432-1912 |
en |
pubs.record-created-at-source-date |
2016-04-07 |
en |
pubs.dimensions-id |
26899864 |
en |