dc.contributor.author |
Sanderson, Leslie E |
en |
dc.contributor.author |
Chien, An-Tzu |
en |
dc.contributor.author |
Astin, Jonathan |
en |
dc.contributor.author |
Crosier, Kathryn |
en |
dc.contributor.author |
Crosier, Philip |
en |
dc.contributor.author |
Hall, Christopher |
en |
dc.date.accessioned |
2018-10-15T02:15:33Z |
en |
dc.date.issued |
2015-11 |
en |
dc.identifier.issn |
0145-305X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/41541 |
en |
dc.description.abstract |
Macrophages are the most functionally heterogenous cells of the hematopoietic system. Given many diseases are underpinned by inappropriate macrophage activation, macrophages have emerged as a therapeutic target to treat disease. A thorough understanding of what controls macrophage activation will likely reveal new pathways that can be manipulated for therapeutic benefit. Live imaging fluorescent macrophages within transgenic zebrafish larvae has provided a valuable window to investigate macrophage behavior in vivo. Here we describe the first transgenic zebrafish line that reports macrophage activation, as evidenced by induced expression of an immunoresponsive gene 1(irg1):EGFP transgene. When combined with existing reporter lines that constitutively mark macrophages, we reveal this unique transgenic line can be used to live image macrophage activation in response to the bacterial endotoxin lipopolysaccharide and xenografted human cancer cells. We anticipate the Tg(irg1:EGFP) line will provide a valuable tool to explore macrophage activation and plasticity in the context of different disease models. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Developmental and comparative immunology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Cell Line, Tumor |
en |
dc.subject |
Macrophages |
en |
dc.subject |
Animals |
en |
dc.subject |
Animals, Genetically Modified |
en |
dc.subject |
Zebrafish |
en |
dc.subject |
Humans |
en |
dc.subject |
Breast Neoplasms |
en |
dc.subject |
Hydro-Lyases |
en |
dc.subject |
Lipopolysaccharides |
en |
dc.subject |
Zebrafish Proteins |
en |
dc.subject |
Green Fluorescent Proteins |
en |
dc.subject |
Transplantation, Heterologous |
en |
dc.subject |
Neoplasm Transplantation |
en |
dc.subject |
Macrophage Activation |
en |
dc.subject |
Larva |
en |
dc.subject |
Female |
en |
dc.subject |
Promoter Regions, Genetic |
en |
dc.title |
An inducible transgene reports activation of macrophages in live zebrafish larvae. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.dci.2015.06.013 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
63 |
en |
pubs.volume |
53 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
26123890 |
en |
pubs.end-page |
69 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
489717 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1879-0089 |
en |
pubs.record-created-at-source-date |
2015-08-13 |
en |
pubs.dimensions-id |
26123890 |
en |