dc.contributor.author |
Radcliff, Fiona |
en |
dc.contributor.author |
Fraser, John |
en |
dc.contributor.author |
Proft, Thomas |
en |
dc.date.accessioned |
2018-10-15T02:55:27Z |
en |
dc.date.issued |
2015-04 |
en |
dc.identifier.issn |
0300-8584 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/41630 |
en |
dc.description.abstract |
Streptococcus pyogenes is a human pathogen which causes a spectrum of diseases ranging from pharyngitis to rheumatic fever, necrotising fasciitis and toxic shock syndrome. Development of a vaccine for S. pyogenes has been confounded both by the diversity of the disease-causing serotypes and the spectre of inadvertently stimulating autoimmunity. The S. pyogenes nuclease A (SpnA) is a recently characterised virulence factor that is highly conserved across strains and expressed during human disease. Deletion of spnA from S. pyogenes results in reduced survival of bacteria in whole human blood and attenuated virulence in a mouse model of infection. Collectively these features suggest that SpnA has potential as a vaccine candidate for S. pyogenes. Mice vaccinated subcutaneously with single or multiple doses of recombinant SpnA emulsified in Incomplete Freund's Adjuvant developed a robust and durable IgG response, including neutralising activity, to this protein. However, vaccination with rSpnA conferred no advantage in terms of lesion development, disease symptoms or colonisation levels after a sub-lethal subcutaneous challenge with S. pyogenes. Anti-SpnA serum IgG responses and neutralising activity were increased in response to challenge, indicating that SpnA is expressed in vivo. SpnA is unlikely to be a suitable antigen for a vaccine against S. pyogenes. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Medical microbiology and immunology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
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dc.subject |
Animals |
en |
dc.subject |
Mice |
en |
dc.subject |
Streptococcus pyogenes |
en |
dc.subject |
Streptococcal Infections |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Deoxyribonucleases |
en |
dc.subject |
Immunoglobulin G |
en |
dc.subject |
Virulence Factors |
en |
dc.subject |
Streptococcal Vaccines |
en |
dc.subject |
Vaccines, Subunit |
en |
dc.subject |
Freund's Adjuvant |
en |
dc.subject |
Antibodies, Bacterial |
en |
dc.subject |
Treatment Outcome |
en |
dc.subject |
Vaccination |
en |
dc.subject |
Injections, Subcutaneous |
en |
dc.subject |
Antibody Formation |
en |
dc.subject |
Female |
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dc.title |
Vaccination with Streptococcus pyogenes nuclease A stimulates a high antibody response but no protective immunity in a mouse model of infection. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s00430-014-0353-2 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
185 |
en |
pubs.volume |
204 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
25119670 |
en |
pubs.end-page |
191 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
449818 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1432-1831 |
en |
pubs.record-created-at-source-date |
2015-03-17 |
en |
pubs.dimensions-id |
25119670 |
en |