dc.contributor.author |
Sutherland, Jessie M |
en |
dc.contributor.author |
Sobinoff, Alexander P |
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dc.contributor.author |
Fraser, Barbara A |
en |
dc.contributor.author |
Redgrove, Kate A |
en |
dc.contributor.author |
Siddall, Nicole A |
en |
dc.contributor.author |
Koopman, Peter |
en |
dc.contributor.author |
Hime, Gary R |
en |
dc.contributor.author |
McLaughlin, Eileen |
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dc.date.accessioned |
2018-10-15T20:50:43Z |
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dc.date.issued |
2018-04 |
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dc.identifier.citation |
Journal of cellular physiology 233(4):3262-3273 Apr 2018 |
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dc.identifier.issn |
0021-9541 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/41757 |
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dc.description.abstract |
RNA-binding proteins (RBP) are important facilitators of post-transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi-2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2. Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalization, identified differential expression and localization of important regulators of transcription, translation, RNA processing, and spermatogenesis. Specifically, the testis-expressed transcription factor, Tbx1, and the piRNA regulator of gamete development, Piwil1, were both found to be targeted for translational repression by MSI2. This study provides key evidence to support a fundamental role for MSI2 in post-transcriptional regulation during male gamete development. |
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dc.format.medium |
Print-Electronic |
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dc.language |
eng |
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dc.relation.ispartofseries |
Journal of cellular physiology |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
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dc.rights |
This is the peer reviewed version of the following article: Sutherland JM, Sobinoff AP, Fraser BA, Redgrove KA, Siddall NA, Koopman P, Hime GR, & McLaughlin EA (2018). RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. Journal of cellular physiology 233(4):3262-3273, which has been published in final form at http://dx.doi.org/10.1002/jcp.26168. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html |
en |
dc.subject |
Spermatids |
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dc.subject |
Animals |
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dc.subject |
Mice, Transgenic |
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dc.subject |
RNA-Binding Proteins |
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dc.subject |
T-Box Domain Proteins |
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dc.subject |
Proteome |
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dc.subject |
RNA, Messenger |
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dc.subject |
Spermatogenesis |
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dc.subject |
Gene Expression Regulation |
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dc.subject |
Models, Biological |
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dc.subject |
Male |
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dc.subject |
Argonaute Proteins |
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dc.title |
RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. |
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dc.type |
Journal Article |
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dc.identifier.doi |
10.1002/jcp.26168 |
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pubs.issue |
4 |
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pubs.begin-page |
3262 |
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pubs.volume |
233 |
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dc.rights.holder |
Copyright: Wiley |
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dc.identifier.pmid |
28884822 |
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pubs.end-page |
3273 |
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pubs.publication-status |
Published |
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dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
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pubs.subtype |
Research Support, Non-U.S. Gov't |
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pubs.subtype |
Journal Article |
en |
pubs.elements-id |
663576 |
en |
dc.identifier.eissn |
1097-4652 |
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pubs.record-created-at-source-date |
2017-09-09 |
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pubs.dimensions-id |
28884822 |
en |