dc.contributor.author |
Gao, Yang |
en |
dc.contributor.author |
Watson, Maureen |
en |
dc.contributor.author |
Callon, Karen |
en |
dc.contributor.author |
Tuari, Donna |
en |
dc.contributor.author |
Dray, Michael |
en |
dc.contributor.author |
Naot, Dorit |
en |
dc.contributor.author |
Amirapu, Satya |
en |
dc.contributor.author |
Munro, Jacob |
en |
dc.contributor.author |
Cornish, Jillian |
en |
dc.contributor.author |
Musson, David |
en |
dc.date.accessioned |
2018-10-16T19:31:52Z |
en |
dc.date.issued |
2018-01 |
en |
dc.identifier.issn |
1932-6254 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/41989 |
en |
dc.description.abstract |
Lactoferrin is a multifunctional glycoprotein with therapeutic potential for bone tissue engineering. The aim of this study was to assess the efficacy of local application of lactoferrin on bone regeneration. Five-millimetre critical-sized defects were created over the right parietal bone in 64 Sprague-Dawley rats. The rats were randomized into four groups: group 1 (n = 20) had empty defects; group 2 (n = 20) had defects grafted with collagen gels (3 mg/ml); group 3 (n = 20) had defects grafted with collagen gels impregnated with bovine lactoferrin (10 μg/gel); and group 4 (n = 4) had sham surgeries (skin and periosteal incisions only). The rats were sacrificed at 4 or 12 weeks post-operatively, and the calvaria were excised and evaluated with micro-CT (Skyscan 1172) followed by histology. The bone volume fraction (BV/TV) was higher in lactoferrin-treated animals at both timepoints, with groups 1, 2, 3 and 4 measuring 10.5 ± 1.1%, 8.6 ± 1.4%, 16.5 ± 0.6% and 24.27 ± 2.6%, respectively, at 4 weeks (P < 0.05); and 12.2 ± 1.3%, 13.6 ± 1.5%, 21.9 ± 1.2% and 29.3 ± 0.8%, respectively, at 12 weeks (P < 0.05). Histological analysis revealed that the newly formed bone within the calvarial defects of all groups was a mixture of woven and lamellar bone, with more bone in the group treated with lactoferrin at both timepoints. Our study demonstrated that local application of lactoferrin significantly increased bone regeneration in a rat critical-sized calvarial defect model. The profound effect of lactoferrin on bone regeneration has therapeutic potential to improve the poor clinical outcomes associated with bony non-union. LF In Vivo JTERM Authors Contributions. Copyright © 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons, Ltd. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Journal of tissue engineering and regenerative medicine |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
en |
dc.subject |
Skull |
en |
dc.subject |
Animals |
en |
dc.subject |
Cattle |
en |
dc.subject |
Rats, Sprague-Dawley |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Lactoferrin |
en |
dc.subject |
Organ Size |
en |
dc.subject |
Bone Regeneration |
en |
dc.subject |
Male |
en |
dc.subject |
X-Ray Microtomography |
en |
dc.title |
Local application of lactoferrin promotes bone regeneration in a rat critical-sized calvarial defect model as demonstrated by micro-CT and histological analysis. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1002/term.2348 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
e620 |
en |
pubs.volume |
12 |
en |
dc.rights.holder |
Copyright: The Authors Journal of Tissue Engineering and Regenerative Medicine |
en |
dc.identifier.pmid |
27860377 |
en |
pubs.end-page |
e626 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
546865 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.org-id |
Ophthalmology Department |
en |
pubs.org-id |
Surgery Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1932-7005 |
en |
pubs.record-created-at-source-date |
2016-11-19 |
en |
pubs.dimensions-id |
27860377 |
en |