dc.contributor.author |
Raos, Brad |
en |
dc.contributor.author |
Doyle, CS |
en |
dc.contributor.author |
Simpson, Miriam |
en |
dc.contributor.author |
Graham, Euan |
en |
dc.contributor.author |
Unsworth, Charles |
en |
dc.date.accessioned |
2018-10-16T23:25:58Z |
en |
dc.date.issued |
2018-02-09 |
en |
dc.identifier.issn |
2045-2322 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/42243 |
en |
dc.description.abstract |
Controlling the spatial distribution of glia and neurons in in vitro culture offers the opportunity to study how cellular interactions contribute to large scale network behaviour. A recently developed approach to cell-patterning uses differential adsorption of animal-serum protein on parylene-C and SiO2 surfaces to enable patterning of neurons and glia. Serum, however, is typically poorly defined and generates reproducibility challenges. Alternative activation methods are highly desirable to enable patterning without relying on animal serum. We take advantage of the innate contrasting surface chemistries of parylene-C and SiO2 to enable selective bonding of polyethylene glycol SiO2 surfaces, i.e. PEGylation, rendering them almost completely repulsive to cell adhesion. As the reagents used in the PEGylation protocol are chemically defined, the reproducibility and batch-to-batch variability complications associated with the used of animal serum are avoided. We report that PEGylated parylene-C/SiO2 substrates achieve a contrast in astrocyte density of 65:1 whereas the standard serum-immersion protocol results in a contrast of 5.6:1. Furthermore, single-cell isolation was significantly improved on PEGylated substrates when astrocytes were grown on close-proximity parylene-C nodes, whereas isolation was limited on serum-activated substrates due tolerance for cell adhesion on serum-adsorbed SiO2 surfaces. |
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dc.format.medium |
Electronic |
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dc.language |
eng |
en |
dc.relation.ispartofseries |
Scientific reports |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/2045-2322/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Astrocytes |
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dc.subject |
Cells, Cultured |
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dc.subject |
Serum |
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dc.subject |
Animals |
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dc.subject |
Humans |
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dc.subject |
Silicon Dioxide |
en |
dc.subject |
Polyethylene Glycols |
en |
dc.subject |
Xylenes |
en |
dc.subject |
Polymers |
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dc.subject |
Reproducibility of Results |
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dc.subject |
Cell Adhesion |
en |
dc.subject |
Microtechnology |
en |
dc.title |
Selective PEGylation of Parylene-C/SiO2 Substrates for Improved Astrocyte Cell Patterning. |
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dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1038/s41598-018-21135-0 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
2754 |
en |
pubs.volume |
8 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
29426929 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
725217 |
en |
pubs.org-id |
Engineering |
en |
pubs.org-id |
Engineering Science |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Pharmacy |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Physics |
en |
dc.identifier.eissn |
2045-2322 |
en |
pubs.record-created-at-source-date |
2018-02-11 |
en |
pubs.dimensions-id |
29426929 |
en |