CLE peptide tri-arabinosylation and peptide domain sequence composition are essential for SUNN-dependent autoregulation of nodulation in Medicago truncatula.

Show simple item record Imin, Nijat en Patel, Neha en Corcilius, Leo en Payne, Richard J en Djordjevic, Michael A en 2018-10-17T00:14:01Z en 2018-04 en
dc.identifier.issn 0028-646X en
dc.identifier.uri en
dc.description.abstract MtCLE12 and MtCLE13 encode CLAVATA3/EMBRYO-SURROUNDING REGION RELATED (CLE) peptides which regulate autoregulation of nodulation (AON) in Medicago through the shoot receptor, SUNN (SUPER NUMERIC NODULES). Genetics suggests RDN1 (ROOT-DETERMINED NODULATION 1) arabinosylates MtCLE12 to enable SUNN perception. The functional structures of MtCLE12 and MtCLE13 peptides, however, remain elusive. We combined genetic and chemical synthesis approaches to determine if glyco-modifications of three nodule-expressed CLE peptides are essential for AON. We also examined how root and shoot applied AON-CLEs inhibit nodulation. MtCLE12, MtCLE13 and MtCLE42 peptides were synthesized with hydroxylation, mono-arabinosylation or tri-arabinosylation (TaP) at proline 7. Only MtCLE12-TaP and MtCLE13-TaP peptides induced AON in wild-type (WT) and rdn1-1, but not in sunn-4. The application of MtCLE13-TaP to cotyledons 1 d before rhizobial inoculation completely inhibited both rhizobial infection and nodulation. By contrast, MtCLE12-TaP induced significant AON without abolishing rhizobial infection. The results indicate that key CLE domain amino acids and TaP modifications to MtCLE12 and MtCLE13 are essential for SUNN-dependent AON. We also show evidence that RDN1 does not tri-arabinosylate MtCLE13. Finally, MtCLE13-TaP can induce a strong AON response in shoots that inhibits the entire symbiotic processes in roots. We present a new model for AON in Medicago. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries The New phytologist en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Medicago truncatula en
dc.subject Arabinose en
dc.subject Peptides en
dc.subject Plant Proteins en
dc.subject Amino Acid Sequence en
dc.subject Structure-Activity Relationship en
dc.subject Glycosylation en
dc.subject Homeostasis en
dc.subject Kinetics en
dc.subject Models, Biological en
dc.subject Plant Root Nodulation en
dc.subject Protein Domains en
dc.title CLE peptide tri-arabinosylation and peptide domain sequence composition are essential for SUNN-dependent autoregulation of nodulation in Medicago truncatula. en
dc.type Journal Article en
dc.identifier.doi 10.1111/nph.15019 en
pubs.issue 1 en
pubs.begin-page 73 en
pubs.volume 218 en
dc.rights.holder Copyright: The author en
pubs.end-page 80 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 726410 en Science en Biological Sciences en
dc.identifier.eissn 1469-8137 en
pubs.record-created-at-source-date 2018-02-03 en
pubs.dimensions-id 29393515 en

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