Phagocytosis of Extracellular Vesicles Extruded From the Placenta by Ovarian Cancer Cells Inhibits Growth of the Cancer Cells.

Show simple item record Chen, Qi en Rutten, Victoria en Cheng, Wei-Tzu en Tong, Mancy en Wei, Jia en Stone, Peter en Ching, Lai-Ming en Chamley, Lawrence en 2018-10-17T01:45:09Z en 2018-03 en
dc.identifier.issn 1048-891X en
dc.identifier.uri en
dc.description.abstract OBJECTIVE:Ovarian cancer is a common gynecological cancer, and parity is negatively associated with the incidence of this disease. This negative association is hypothesized to be due in part to shifting the balance of estrogen and progesterone toward more progesterone and reduced ovulation during pregnancy. However, studies suggested that parity is also associated with estrogen-independent gynecological cancers suggesting balance of hormones may not be the only protective factor. Extracellular vesicles (EVs) play an important role in cell-to-cell communication in physiological and pathological conditions. During pregnancy, large amounts of EVs are extruded from the placenta, and they seem to be involved in the remarkable adaptation of a woman's body to normal pregnancy. We hypothesized that EVs extruded from the placenta play a role in this protective effect. METHODS:Placental EVs were collected from first-trimester placentae, and cancer cell EVs were isolated from ovarian cancer cells. The EVs were exposed to ovarian cancer cells for 48 hours. The proliferation of cancer cells and the cell cycle were measured. In addition, phagocytosis of deported placental EVs by cancer cells was also measured. RESULTS:The proliferation of cancer cells was significantly reduced by treatment with placental EVs (P = 0.001, analysis of variance), but not EVs from monocytes (P = 0.195), compared with untreated cancer cells. Furthermore, placental EVs also prevented the proliferation of cancer cells induced by cancer cell-derived EVs (P = 0.001). This inhibition of proliferation of ovarian cancer cells was partially due to phagocytosis of placental EVs by cancer cells. Phagocytosis of placental EVs delayed progression through the cell cycle. Calreticulin, a phagocytic "eat me" signal carried by placental EVs significantly inhibited ovarian cancer growth (P = 0.001). CONCLUSIONS:Our data demonstrated that EVs extruded from the placenta prevented ovarian cancer cell growth by a mechanism that involved delaying progression of the cell cycle after phagocytosis of the EVs. en
dc.format.medium Print en
dc.language eng en
dc.relation.ispartofseries International journal of gynecological cancer : official journal of the International Gynecological Cancer Society en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Cell Line, Tumor en
dc.subject Placenta en
dc.subject Humans en
dc.subject Ovarian Neoplasms en
dc.subject Calreticulin en
dc.subject Recombinant Proteins en
dc.subject Cell Cycle en
dc.subject Cell Proliferation en
dc.subject Phagocytosis en
dc.subject Pregnancy en
dc.subject Female en
dc.subject Extracellular Vesicles en
dc.subject THP-1 Cells en
dc.title Phagocytosis of Extracellular Vesicles Extruded From the Placenta by Ovarian Cancer Cells Inhibits Growth of the Cancer Cells. en
dc.type Journal Article en
dc.identifier.doi 10.1097/igc.0000000000001140 en
pubs.issue 3 en
pubs.begin-page 545 en
pubs.volume 28 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 29040188 en
pubs.end-page 552 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 701917 en Medical and Health Sciences en Medical Sciences en Auckland Cancer Research en School of Medicine en Obstetrics and Gynaecology en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1525-1438 en
pubs.record-created-at-source-date 2017-10-18 en
pubs.dimensions-id 29040188 en

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