dc.contributor.author |
Maia, F Raquel |
en |
dc.contributor.author |
Musson, David |
en |
dc.contributor.author |
Naot, Dorit |
en |
dc.contributor.author |
da Silva, Lucilia P |
en |
dc.contributor.author |
Bastos, Ana R |
en |
dc.contributor.author |
Costa, João B |
en |
dc.contributor.author |
Oliveira, Joaquim M |
en |
dc.contributor.author |
Correlo, Vitor M |
en |
dc.contributor.author |
Reis, Rui L |
en |
dc.contributor.author |
Cornish, Jillian |
en |
dc.date.accessioned |
2018-10-17T02:57:43Z |
en |
dc.date.issued |
2018-03-16 |
en |
dc.identifier.citation |
Biomedical materials (Bristol, England) 13(3):035012 16 Mar 2018 |
en |
dc.identifier.issn |
1748-6041 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/42552 |
en |
dc.description.abstract |
Bone tissue engineering with cell-scaffold constructs has been attracting a lot of attention, in particular as a tool for the efficient guiding of new tissue formation. However, the majority of the current strategies used to evaluate novel biomaterials focus on osteoblasts and bone formation, while osteoclasts are often overlooked. Consequently, there is limited knowledge on the interaction between osteoclasts and biomaterials. In this study, the ability of spongy-like gellan gum and hydroxyapatite-reinforced gellan gum hydrogels to support osteoclastogenesis was investigated in vitro. First, the spongy-like gellan gum and hydroxyapatite-reinforced gellan gum hydrogels were characterized in terms of microstructure, water uptake and mechanical properties. Then, bone marrow cells isolated from the long bones of mice and cultured in spongy-like hydrogels were treated with 1,25-dihydroxyvitamin D3 to promote osteoclastogenesis. It was shown that the addition of HAp to spongy-like gellan gum hydrogels enables the formation of larger pores and thicker walls, promoting an increase in stiffness. Hydroxyapatite-reinforced spongy-like gellan gum hydrogels support the formation of the aggregates of tartrate-resistant acid phosphatase-stained cells and the expression of genes encoding DC-STAMP and Cathepsin K, suggesting the differentiation of bone marrow cells into pre-osteoclasts. The hydroxyapatite-reinforced spongy-like gellan gum hydrogels developed in this work show promise for future use in bone tissue scaffolding applications. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Biomedical materials (Bristol, England) |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights |
This is an author-created, un-copyedited version of an article accepted for publication/published
in Biomedical materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or
any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/1748-605x/aaaf29 |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licences/by-nc-nd/3.0 |
en |
dc.subject |
Bone and Bones |
en |
dc.subject |
Bone Marrow Cells |
en |
dc.subject |
Cells, Cultured |
en |
dc.subject |
Osteoclasts |
en |
dc.subject |
Osteoblasts |
en |
dc.subject |
Animals |
en |
dc.subject |
Mice |
en |
dc.subject |
Durapatite |
en |
dc.subject |
Calcitriol |
en |
dc.subject |
Polysaccharides, Bacterial |
en |
dc.subject |
Biocompatible Materials |
en |
dc.subject |
Hydrogels |
en |
dc.subject |
Tissue Engineering |
en |
dc.subject |
Cell Differentiation |
en |
dc.subject |
Cell Survival |
en |
dc.subject |
Male |
en |
dc.subject |
Tissue Scaffolds |
en |
dc.subject |
Cathepsin K |
en |
dc.title |
Differentiation of osteoclast precursors on gellan gum-based spongy-like hydrogels for bone tissue engineering. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1088/1748-605x/aaaf29 |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
035012 |
en |
pubs.volume |
13 |
en |
dc.rights.holder |
Copyright: IOP Publishing Ltd. |
en |
dc.identifier.pmid |
29442071 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
725281 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1748-605X |
en |
pubs.record-created-at-source-date |
2018-02-15 |
en |
pubs.dimensions-id |
29442071 |
en |