Analysis of αSMA-labeled progenitor cell commitment identifies notch signaling as an important pathway in fracture healing.

Show simple item record Matthews, Brya en Grcevic, Danka en Wang, Liping en Hagiwara, Yusuke en Roguljic, Hrvoje en Joshi, Pujan en Shin, Dong-Guk en Adams, Douglas J en Kalajzic, Ivo en 2018-10-18T00:19:03Z en 2014-01 en
dc.identifier.issn 0884-0431 en
dc.identifier.uri en
dc.description.abstract Fracture healing is a regenerative process that involves coordinated responses of many cell types, but characterization of the roles of specific cell populations in this process has been limited. We have identified alpha smooth muscle actin (αSMA) as a marker of a population of mesenchymal progenitor cells in the periosteum that contributes to osteochondral elements during fracture healing. Using a lineage tracing approach, we labeled αSMA-expressing cells, and characterized changes in the periosteal population during the early stages of fracture healing by histology, flow cytometry, and gene expression profiling. In response to fracture, the αSMA-labeled population expanded and began to differentiate toward the osteogenic and chondrogenic lineages. The frequency of mesenchymal progenitor cell markers such as Sca1 and PDGFRα increased after fracture. By 6 days after fracture, genes involved in matrix production and remodeling were elevated. In contrast, genes associated with muscle contraction and Notch signaling were downregulated after fracture. We confirmed that activating Notch signaling in αSMA-labeled cells inhibited differentiation into osteogenic and adipogenic lineages in vitro and ectopic bone formation in vivo. By characterizing changes in a selected αSMA-labeled progenitor cell population during fracture callus formation, we have shown that modulation of Notch signaling may determine osteogenic potential of αSMA-expressing progenitor cells during bone healing. en
dc.format.medium Print en
dc.language eng en
dc.relation.ispartofseries Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Stem Cells en
dc.subject Animals en
dc.subject Mice, Transgenic en
dc.subject Mice en
dc.subject Actins en
dc.subject Staining and Labeling en
dc.subject Fracture Healing en
dc.subject Signal Transduction en
dc.subject Osteogenesis en
dc.subject Receptors, Notch en
dc.subject Cell Tracking en
dc.title Analysis of αSMA-labeled progenitor cell commitment identifies notch signaling as an important pathway in fracture healing. en
dc.type Journal Article en
dc.identifier.doi 10.1002/jbmr.2140 en
pubs.issue 5 en
pubs.begin-page 1283 en
pubs.volume 29 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 24190076 en
pubs.end-page 1294 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.subtype Research Support, N.I.H., Extramural en
pubs.elements-id 666126 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en
dc.identifier.eissn 1523-4681 en
pubs.record-created-at-source-date 2014-04-23 en
pubs.dimensions-id 24190076 en

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