dc.contributor.author |
Vidovic, I |
en |
dc.contributor.author |
Banerjee, A |
en |
dc.contributor.author |
Fatahi, R |
en |
dc.contributor.author |
Matthews, Brya |
en |
dc.contributor.author |
Dyment, NA |
en |
dc.contributor.author |
Kalajzic, I |
en |
dc.contributor.author |
Mina, M |
en |
dc.date.accessioned |
2018-10-18T00:19:40Z |
en |
dc.date.issued |
2017-03 |
en |
dc.identifier.issn |
0022-0345 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/42772 |
en |
dc.description.abstract |
The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the αSMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of αSMA-tdTomato+ cells to a small number of newly formed odontoblasts during primary dentinogenesis. Using an experimental pulp exposure model in molars to induce reparative dentinogenesis, we demonstrate the contribution of αSMA-tdTomato+ cells to cells secreting reparative dentin. Our results demonstrate that αSMA-tdTomato+ cells differentiated into Col2.3-GFP+ cells composed of both Dspp+ odontoblasts and Bsp+ osteoblasts. Our findings identify a population of mesenchymal progenitor cells capable of giving rise to a second generation of odontoblasts during reparative dentinogenesis. This population also makes a small contribution to odontoblasts during primary dentinogenesis. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Journal of dental research |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Odontoblasts |
en |
dc.subject |
Osteoblasts |
en |
dc.subject |
Molar |
en |
dc.subject |
Dental Pulp |
en |
dc.subject |
Animals |
en |
dc.subject |
Mice, Transgenic |
en |
dc.subject |
Mice |
en |
dc.subject |
Actins |
en |
dc.subject |
Immunohistochemistry |
en |
dc.subject |
Cell Differentiation |
en |
dc.subject |
Dentinogenesis |
en |
dc.subject |
Transgenes |
en |
dc.subject |
Mesenchymal Stromal Cells |
en |
dc.title |
αSMA-Expressing Perivascular Cells Represent Dental Pulp Progenitors In Vivo. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1177/0022034516678208 |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
323 |
en |
pubs.volume |
96 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
27834664 |
en |
pubs.end-page |
330 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.subtype |
Research Support, N.I.H., Extramural |
en |
pubs.elements-id |
660149 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
dc.identifier.eissn |
1544-0591 |
en |
pubs.record-created-at-source-date |
2016-11-12 |
en |
pubs.dimensions-id |
27834664 |
en |