dc.contributor.author |
Dragunow, Michael |
en |
dc.contributor.author |
Xu, Ruian |
en |
dc.contributor.author |
Walton, M |
en |
dc.contributor.author |
Woodgate, Ann-Marie |
en |
dc.contributor.author |
Lawlor, Patricia |
en |
dc.contributor.author |
MacGibbon, Geraldine |
en |
dc.contributor.author |
Young, Deborah |
en |
dc.contributor.author |
Gibbons, Hannah |
en |
dc.contributor.author |
Lipski, Janusz |
en |
dc.contributor.author |
Muravlev, A |
en |
dc.contributor.author |
Pearson, Andree |
en |
dc.contributor.author |
During, Matthew |
en |
dc.date.accessioned |
2018-10-18T00:31:19Z |
en |
dc.date.issued |
2000 |
en |
dc.identifier.issn |
0169-328X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/42784 |
en |
dc.description.abstract |
We investigated the function of c-Jun in PC12 cells by transfecting them with a plasmid containing a c-Jun cDNA transcription cassette. Transfected cells expressed high levels of c-Jun mRNA and protein and demonstrated an increase in both AP-1 DNA binding and gene activation. The c-Jun over-expressing cells showed marked neurite outgrowth but no evidence of spontaneous cell death. In fact, c-Jun over-expressing cells were more resistant to okadaic acid-induced apoptosis. The process outgrowth was not indicative of a full neuronal differentiation response as the transfected PC12 cells did not display action potentials when examined with whole-cell patch-clamping. The phosphorylation of c-Jun on serine 73 appears to be important for this neurite sprouting effect as mutagenesis at this site reduced sprouting whereas a serine 63 mutant tended to increase sprouting. Thus, in PC12 cells c-Jun expression does not induce apoptosis, but rather functions as a neurite outgrowth and neuronal survival signal. |
en |
dc.publisher |
Elsevier |
en |
dc.relation.ispartofseries |
Molecular Brain Research |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
c-Jun promotes neurite outgrowth and survival in PC12 cells |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/s0169-328x(00)00191-1 |
en |
pubs.issue |
1-2 |
en |
pubs.begin-page |
20 |
en |
pubs.volume |
83 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
11072092 |
en |
pubs.end-page |
33 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
44621 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Pharmacology |
en |
pubs.org-id |
Physiology Division |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
11072092 |
en |