c-Jun promotes neurite outgrowth and survival in PC12 cells

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dc.contributor.author Dragunow, Michael en
dc.contributor.author Xu, Ruian en
dc.contributor.author Walton, M en
dc.contributor.author Woodgate, Ann-Marie en
dc.contributor.author Lawlor, Patricia en
dc.contributor.author MacGibbon, Geraldine en
dc.contributor.author Young, Deborah en
dc.contributor.author Gibbons, Hannah en
dc.contributor.author Lipski, Janusz en
dc.contributor.author Muravlev, A en
dc.contributor.author Pearson, Andree en
dc.contributor.author During, Matthew en
dc.date.accessioned 2018-10-18T00:31:19Z en
dc.date.issued 2000 en
dc.identifier.issn 0169-328X en
dc.identifier.uri http://hdl.handle.net/2292/42784 en
dc.description.abstract We investigated the function of c-Jun in PC12 cells by transfecting them with a plasmid containing a c-Jun cDNA transcription cassette. Transfected cells expressed high levels of c-Jun mRNA and protein and demonstrated an increase in both AP-1 DNA binding and gene activation. The c-Jun over-expressing cells showed marked neurite outgrowth but no evidence of spontaneous cell death. In fact, c-Jun over-expressing cells were more resistant to okadaic acid-induced apoptosis. The process outgrowth was not indicative of a full neuronal differentiation response as the transfected PC12 cells did not display action potentials when examined with whole-cell patch-clamping. The phosphorylation of c-Jun on serine 73 appears to be important for this neurite sprouting effect as mutagenesis at this site reduced sprouting whereas a serine 63 mutant tended to increase sprouting. Thus, in PC12 cells c-Jun expression does not induce apoptosis, but rather functions as a neurite outgrowth and neuronal survival signal. en
dc.publisher Elsevier en
dc.relation.ispartofseries Molecular Brain Research en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title c-Jun promotes neurite outgrowth and survival in PC12 cells en
dc.type Journal Article en
dc.identifier.doi 10.1016/s0169-328x(00)00191-1 en
pubs.issue 1-2 en
pubs.begin-page 20 en
pubs.volume 83 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 11072092 en
pubs.end-page 33 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 44621 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Pharmacology en
pubs.org-id Physiology Division en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 11072092 en

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