dc.contributor.author |
Zhang, Wenli |
en |
dc.contributor.author |
Li, Caibin |
en |
dc.contributor.author |
Baguley, Bruce |
en |
dc.contributor.author |
Zhou, Fang |
en |
dc.contributor.author |
Zhou, Weisai |
en |
dc.contributor.author |
Shaw, John |
en |
dc.contributor.author |
Wang, Zhen |
en |
dc.contributor.author |
Wu, Zimei |
en |
dc.contributor.author |
Liu, Jianping |
en |
dc.date.accessioned |
2018-10-18T01:58:06Z |
en |
dc.date.issued |
2016-12 |
en |
dc.identifier.issn |
0003-2697 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/42827 |
en |
dc.description.abstract |
To obtain a multicellular MCF-7 spheroid model to mimic the three-dimensional (3D) of tumors, the microwell liquid overlay (A) and hanging-drop/agar (B) methods were first compared for their technical parameters. Then a method for embedding spheroids within collagen was optimized. For method A, centrifugation assisted cells form irregular aggregates but not spheroids. For method B, an extended sedimentation period of over 24 h for cell suspensions and increased viscosity of the culture medium using methylcellulose were necessary to harvest a dense and regular cell spheroid. When the number was less than 5000 cells/drop, embedded spheroids showed no tight cores and higher viability than the unembedded. However, above 5000 cells/drop, cellular viability of embedded spheroids was not significantly different from unembedded spheroids and cells invading through the collagen were in a sun-burst pattern with tight cores. Propidium Iodide staining indicated that spheroids had necrotic cores. The doxorubicin cytotoxicity demonstrated that spheroids were less susceptible to DOX than their monolayer cells. A reliable and reproducible method for embedding spheroids using the hanging-drop/agarose method within collagen is described herein. The cell culture model can be used to guide experimental manipulation of 3D cell cultures and to evaluate anticancer drug efficacy. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Analytical biochemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Spheroids, Cellular |
en |
dc.subject |
Humans |
en |
dc.subject |
Doxorubicin |
en |
dc.subject |
Cell Culture Techniques |
en |
dc.subject |
Biomimetics |
en |
dc.subject |
Cell Survival |
en |
dc.subject |
Female |
en |
dc.subject |
MCF-7 Cells |
en |
dc.title |
Optimization of the formation of embedded multicellular spheroids of MCF-7 cells: How to reliably produce a biomimetic 3D model. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.ab.2016.10.004 |
en |
pubs.begin-page |
47 |
en |
pubs.volume |
515 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
27717854 |
en |
pubs.end-page |
54 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
542872 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Pharmacy |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1096-0309 |
en |
pubs.record-created-at-source-date |
2016-10-08 |
en |
pubs.dimensions-id |
27717854 |
en |