Chromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure

Show simple item record Grand, RS en Pichugina, T en Gehlen, LR en Jones, Mary Beatrix en Tsai, Peter en Allison, Jane en Martienssen, R en O Sullivan, JM en 2018-10-19T03:06:35Z en 2014-11-10 en
dc.identifier.citation Nucleic Acids Research 42(20):12585-12599 10 Nov 2014 en
dc.identifier.issn 0305-1048 en
dc.identifier.uri en
dc.description.abstract Successful progression through the cell cycle requires spatial and temporal regulation of gene transcript levels and the number, positions and condensation levels of chromosomes. Here we present a high resolution survey of genome interactions in Schizosaccharomyces pombe using synchronized cells to investigate cell cycle dependent changes in genome organization and transcription. Cell cycle dependent interactions were captured between and within S. pombe chromosomes. Known features of genome organization (e.g. the clustering of telomeres and retrotransposon long terminal repeats (LTRs)) were observed throughout the cell cycle. There were clear correlations between transcript levels and chromosomal interactions between genes, consistent with a role for interactions in transcriptional regulation at specific stages of the cell cycle. In silico reconstructions of the chromosome organization within the S. pombe nuclei were made by polymer modeling. These models suggest that groups of genes with high and low, or differentially regulated transcript levels have preferred positions within the S. pombe nucleus. We conclude that the S. pombe nucleus is spatially divided into functional sub-nuclear domains that correlate with gene activity. The observation that chromosomal interactions are maintained even when chromosomes are fully condensed in M phase implicates genome organization in epigenetic inheritance and bookmarking. en
dc.language English en
dc.publisher Oxford University Press en
dc.relation.ispartofseries Nucleic Acids Research en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.subject Science & Technology en
dc.subject Life Sciences & Biomedicine en
dc.subject Biochemistry & Molecular Biology en
dc.subject POL III GENES en
dc.subject REPLICATION en
dc.subject CHROMATIN en
dc.subject BOOKMARKING en
dc.subject REORGANIZATION en
dc.title Chromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure en
dc.type Journal Article en
dc.identifier.doi 10.1093/nar/gku965 en
pubs.issue 20 en
pubs.begin-page 12585 en
pubs.volume 42 en
dc.rights.holder Copyright: The authors en
dc.identifier.pmid 25342201 en
pubs.end-page 12599 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 459487 en Liggins Institute en Medical and Health Sciences en School of Medicine en Obstetrics and Gynaecology en Science en Biological Sciences en Statistics en
dc.identifier.eissn 1362-4962 en
dc.identifier.pii gku965 en
pubs.record-created-at-source-date 2018-05-08 en
pubs.dimensions-id 25342201 en

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