Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays.

Show simple item record Macdonald, NP en Zhu, F en Hall, Christopher en Reboud, J en Crosier, Philip en Patton, EE en Wlodkowic, D en Cooper, JM en 2018-11-05T22:54:20Z en 2016-01 en
dc.identifier.citation Lab on a chip 16(2):291-297 Jan 2016 en
dc.identifier.issn 1473-0197 en
dc.identifier.uri en
dc.description.abstract 3D printing has emerged as a rapid and cost-efficient manufacturing technique to enable the fabrication of bespoke, complex prototypes. If the technology is to have a significant impact in biomedical applications, such as drug discovery and molecular diagnostics, the devices produced must be biologically compatible to enable their use with established reference assays and protocols. In this work we demonstrate that we can adapt the Fish Embryo Test (FET) as a new method to quantify the toxicity of 3D printed microfluidic devices. We assessed the biocompatibility of four commercially available 3D printing polymers (VisiJetCrystal EX200, Watershed 11122XC, Fototec SLA 7150 Clear and ABSplus P-430), through the observation of key developmental markers in the developing zebrafish embryos. Results show all of the photopolymers to be highly toxic to the embryos, resulting in fatality, although we do demonstrate that post-printing treatment of Fototec 7150 makes it suitable for zebrafish culture within the FET. en
dc.format.medium Print en
dc.language eng en
dc.relation.ispartofseries Lab on a chip en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.rights.uri en
dc.subject Animals en
dc.subject Zebrafish en
dc.subject Polymers en
dc.subject Biocompatible Materials en
dc.subject Microfluidic Analytical Techniques en
dc.subject Toxicity Tests en
dc.subject Photochemical Processes en
dc.subject Printing, Three-Dimensional en
dc.title Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays. en
dc.type Journal Article en
dc.identifier.doi 10.1039/c5lc01374g en
pubs.issue 2 en
pubs.begin-page 291 en
pubs.volume 16 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 26646354 en
pubs.end-page 297 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 515056 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1473-0189 en
pubs.record-created-at-source-date 2015-12-10 en
pubs.dimensions-id 26646354 en

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