NMR studies of the non-haem Fe(II) and 2-oxoglutarate-dependent oxygenases

Mbenza, Naasson Mbambi; Vadakkedath, Praveen George; McGillivray, Duncan; Leung, KH

dc.contributor.author	Mbenza, Naasson Mbambi	en
dc.contributor.author	Vadakkedath, Praveen George	en
dc.contributor.author	McGillivray, Duncan	en
dc.contributor.author	Leung, KH	en
dc.date.accessioned	2018-11-06T21:45:20Z	en
dc.date.issued	2017	en
dc.identifier.issn	1873-3344	en
dc.identifier.uri	http://hdl.handle.net/2292/44046	en
dc.description.abstract	The non-haem Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenases belong to a superfamily of structurally-related enzymes that play important biological roles in plants, microorganisms and animals. Structural, mechanistic and functional studies of 2OG oxygenases require efficient and effective biophysical tools. Nuclear magnetic resonance (NMR) spectroscopy is a useful tool to study this enzyme superfamily. It has been applied to obtain information about enzyme kinetics, identify and characterise 2OG oxygenase-catalysed oxidation products, elucidate the catalytic mechanism, monitor ligand binding and study protein dynamics. This review summarises the types of information that NMR spectroscopy can provide in the studies of 2OG oxygenases, highlights the advantages of the technique and describes its drawbacks.	en
dc.publisher	Elsevier	en
dc.relation.ispartofseries	Journal of Inorganic Biochemistry	en
dc.rights	Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.	en
dc.rights.uri	https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm	en
dc.title	NMR studies of the non-haem Fe(II) and 2-oxoglutarate-dependent oxygenases	en
dc.type	Journal Article	en
dc.identifier.doi	10.1016/j.jinorgbio.2017.08.032	en
dc.rights.holder	Copyright: The author	en
dc.identifier.pmid	28893416	en
pubs.author-url	http://doi.org/10.1016/j.jinorgbio.2017.08.032	en
dc.rights.accessrights	http://purl.org/eprint/accessRights/RestrictedAccess	en
pubs.subtype	Review	en
pubs.elements-id	659715	en
pubs.org-id	Academic Services	en
pubs.org-id	Examinations	en
pubs.org-id	Science	en
pubs.org-id	Chemistry	en
pubs.record-created-at-source-date	2017-09-06	en
pubs.online-publication-date	2017-09-04	en
pubs.dimensions-id	28893416	en