dc.contributor.author |
Shchepinova, Maria M |
en |
dc.contributor.author |
Cairns, Andrew G |
en |
dc.contributor.author |
Prime, Tracy A |
en |
dc.contributor.author |
Logan, Angela |
en |
dc.contributor.author |
James, Andrew M |
en |
dc.contributor.author |
Hall, Andrew R |
en |
dc.contributor.author |
Vidoni, Sara |
en |
dc.contributor.author |
Arndt, Sabine |
en |
dc.contributor.author |
Caldwell, Stuart T |
en |
dc.contributor.author |
Prag, Hiran A |
en |
dc.contributor.author |
Pell, Victoria R |
en |
dc.contributor.author |
Krieg, Thomas |
en |
dc.contributor.author |
Mulvey, John F |
en |
dc.contributor.author |
Yadav, Pooja |
en |
dc.contributor.author |
Cobley, James N |
en |
dc.contributor.author |
Bright, Thomas P |
en |
dc.contributor.author |
Senn, Hans M |
en |
dc.contributor.author |
Anderson, Robert |
en |
dc.contributor.author |
Murphy, Michael P |
en |
dc.contributor.author |
Hartley, Richard C |
en |
dc.date.accessioned |
2018-11-12T20:24:15Z |
en |
dc.date.issued |
2017-10 |
en |
dc.identifier.citation |
Cell Chemical Biology 24(10):1285-1298.e12 19 Oct 2017 |
en |
dc.identifier.issn |
2451-9456 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/44123 |
en |
dc.description.abstract |
Mitochondrial superoxide (O2⋅-) underlies much oxidative damage and redox signaling. Fluorescent probes can detect O2⋅-, but are of limited applicability in vivo, while in cells their usefulness is constrained by side reactions and DNA intercalation. To overcome these limitations, we developed a dual-purpose mitochondrial O2⋅- probe, MitoNeoD, which can assess O2⋅- changes in vivo by mass spectrometry and in vitro by fluorescence. MitoNeoD comprises a O2⋅--sensitive reduced phenanthridinium moiety modified to prevent DNA intercalation, as well as a carbon-deuterium bond to enhance its selectivity for O2⋅- over non-specific oxidation, and a triphenylphosphonium lipophilic cation moiety leading to the rapid accumulation within mitochondria. We demonstrated that MitoNeoD was a versatile and robust probe to assess changes in mitochondrial O2⋅- from isolated mitochondria to animal models, thus offering a way to examine the many roles of mitochondrial O2⋅- production in health and disease. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Cell chemical biology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Cell Line |
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dc.subject |
Mitochondria |
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dc.subject |
Animals |
en |
dc.subject |
Mice |
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dc.subject |
Superoxides |
en |
dc.subject |
DNA |
en |
dc.subject |
Molecular Probes |
en |
dc.subject |
Nucleic Acid Conformation |
en |
dc.subject |
Biological Transport |
en |
dc.subject |
Oxidation-Reduction |
en |
dc.subject |
Models, Molecular |
en |
dc.subject |
Male |
en |
dc.subject |
Mass Spectrometry |
en |
dc.title |
MitoNeoD: A Mitochondria-Targeted Superoxide Probe. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.chembiol.2017.08.003 |
en |
pubs.issue |
10 |
en |
pubs.begin-page |
1285 |
en |
pubs.volume |
24 |
en |
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
28890317 |
en |
pubs.end-page |
1298.e12 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
668627 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Auckland Cancer Research |
en |
dc.identifier.eissn |
2451-9448 |
en |
pubs.record-created-at-source-date |
2017-09-12 |
en |
pubs.dimensions-id |
28890317 |
en |