dc.contributor.author |
Galinsky, Robert |
en |
dc.contributor.author |
Davidson, Joanne |
en |
dc.contributor.author |
Lear, Christopher |
en |
dc.contributor.author |
Bennet, Laura |
en |
dc.contributor.author |
Green, Colin |
en |
dc.contributor.author |
Gunn, Alistair |
en |
dc.date.accessioned |
2018-11-13T22:12:41Z |
en |
dc.date.issued |
2017-07-24 |
en |
dc.identifier.citation |
Scientific Reports 7:1-11 Article number 6304 24 Jul 2017 |
en |
dc.identifier.issn |
2045-2322 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/44194 |
en |
dc.description.abstract |
Basal ganglia injury at term remains a major cause of disability, such as cerebral palsy. In this study we tested the hypotheses that blockade of astrocytic connexin hemichannels with a mimetic peptide would improve survival of striatal phenotypic neurons after global cerebral ischaemia in term-equivalent fetal sheep, and that neuronal survival would be associated with electrophysiological recovery. Fetal sheep (0.85 gestation) were randomly assigned to receive a short or long (1 or 25 h) intracerebroventricular infusion of a mimetic peptide or vehicle, starting 90 minutes after 30 minutes of cerebral ischaemia. Sheep were killed 7 days after ischaemia. Cerebral ischaemia was associated with reduced numbers of calbindin-28k, calretinin, parvalbumin and GAD positive striatal neurons (P < 0.05 ischaemia + vehicle, n = 6 vs. sham ischaemia, n = 6) but not ChAT or nNOS positive neurons. Short infusion of peptide (n = 6) did not significantly improve survival of any striatal phenotype. Long infusion of peptide (n = 6) was associated with increased survival of calbindin-28k, calretinin, parvalbumin and GAD positive neurons (P < 0.05 vs. ischaemia + vehicle). Neurophysiological recovery was associated with improved survival of calbindin-28k, calretinin and parvalbumin positive striatal neurons (P < 0.05 for all). In conclusion, connexin hemichannel blockade after cerebral ischaemia in term-equivalent fetal sheep improves survival of striatal GABA-ergic neurons. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Scientific reports |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Corpus Striatum |
en |
dc.subject |
Animals |
en |
dc.subject |
Sheep |
en |
dc.subject |
Brain Ischemia |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Peptides |
en |
dc.subject |
Connexin 43 |
en |
dc.subject |
Random Allocation |
en |
dc.subject |
Pregnancy |
en |
dc.subject |
Female |
en |
dc.subject |
Infusions, Intraventricular |
en |
dc.subject |
GABAergic Neurons |
en |
dc.subject |
Calbindin 1 |
en |
dc.subject |
Calbindin 2 |
en |
dc.subject |
Biological Mimicry |
en |
dc.title |
Connexin hemichannel blockade improves survival of striatal GABA-ergic neurons after global cerebral ischaemia in term-equivalent fetal sheep. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1038/s41598-017-06683-1 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
6304 |
en |
pubs.volume |
7 |
en |
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
28740229 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
640766 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Physiology Division |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
2045-2322 |
en |
pubs.record-created-at-source-date |
2017-07-26 |
en |
pubs.dimensions-id |
28740229 |
en |