dc.contributor.author |
Dalbeth, Nicola |
en |
dc.contributor.author |
Pool, Bregtina |
en |
dc.contributor.author |
Chhana, Ashika |
en |
dc.contributor.author |
Lin, Jianming |
en |
dc.contributor.author |
Tay, Mei Lin |
en |
dc.contributor.author |
Tan, Paul |
en |
dc.contributor.author |
Callon, Karen |
en |
dc.contributor.author |
Naot, Dorit |
en |
dc.contributor.author |
Horne, Anne |
en |
dc.contributor.author |
Drake, Jill |
en |
dc.contributor.author |
Gamble, Gregory |
en |
dc.contributor.author |
Reid, Ian |
en |
dc.contributor.author |
Grey, Andrew |
en |
dc.contributor.author |
Stamp, Lisa K |
en |
dc.contributor.author |
Cornish, Jillian |
en |
dc.date.accessioned |
2018-11-15T02:20:35Z |
en |
dc.date.issued |
2018-01 |
en |
dc.identifier.issn |
0171-967X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/44296 |
en |
dc.description.abstract |
INTRODUCTION:Numerous observational studies have reported that serum urate concentration positively correlates with bone density and reduced risk of fractures. The aim of this study was to examine whether soluble urate directly influences bone remodelling. METHODS:In laboratory studies, the in vitro effects of soluble urate were examined in osteoclast, osteoblast and osteocyte assays at a range of urate concentrations consistent with those typically observed in humans (up to 0.70 mmol/L). The clinical relevance of the in vitro assay findings was assessed using serial procollagen-1 N-terminal propeptide (P1NP) and Month 12 bone density data from a randomised controlled trial of allopurinol dose escalation in people with gout. RESULTS:Addition of urate in the RAW264.7 cell osteoclastogenesis assay led to small increases in osteoclast formation (ANOVA p = 0.018), but no significant difference in bone resorption. No significant effects on osteoclast number or activity were observed in primary cell osteoclastogenesis or resorption assays. Addition of urate did not alter viability or function in MC3T3-E1 pre-osteoblast, primary human osteoblast, or MLO-Y4 osteocyte assays. In the clinical trial analysis, reducing serum urate over a 12 month period by allopurinol dose escalation did not lead to significant changes in P1NP or differences in bone mineral density. CONCLUSION:Addition of soluble urate at physiological concentrations does not influence bone remodelling in vitro. These data, together with clinical trial data showing no effect of urate-lowering on P1NP or bone density, do not support a direct role for urate in influencing bone remodelling. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Calcified tissue international |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Bone and Bones |
en |
dc.subject |
Osteoclasts |
en |
dc.subject |
Osteocytes |
en |
dc.subject |
Humans |
en |
dc.subject |
Bone Resorption |
en |
dc.subject |
Uric Acid |
en |
dc.subject |
Bone Remodeling |
en |
dc.subject |
Cell Differentiation |
en |
dc.subject |
Osteogenesis |
en |
dc.title |
Lack of Evidence that Soluble Urate Directly Influences Bone Remodelling: A Laboratory and Clinical Study. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s00223-017-0328-6 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
73 |
en |
pubs.volume |
102 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
29018897 |
en |
pubs.end-page |
84 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
692488 |
en |
pubs.org-id |
Academic Services |
en |
pubs.org-id |
Examinations |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Anatomy and Medical Imaging |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1432-0827 |
en |
pubs.record-created-at-source-date |
2017-10-12 |
en |
pubs.dimensions-id |
29018897 |
en |