Preclinical development of MGO Manuka Honey microemulsion for blepharitis management.

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dc.contributor.author Craig, Jennifer en
dc.contributor.author Rupenthal, Ilva en
dc.contributor.author Seyfoddin, Ali en
dc.contributor.author Cheung, Isabella MY en
dc.contributor.author Uy, Benedict en
dc.contributor.author Wang, Michael en
dc.contributor.author Watters, Grant A en
dc.contributor.author Swift, Simon en
dc.date.accessioned 2018-11-18T23:21:24Z en
dc.date.issued 2017-01 en
dc.identifier.citation BMJ open ophthalmology 1(1):e000065 Jan 201 en
dc.identifier.issn 2397-3269 en
dc.identifier.uri http://hdl.handle.net/2292/44407 en
dc.description.abstract To evaluate the in vitro antimicrobial effects of cyclodextrin-complexed and uncomplexed Manuka honey on bacteria commonly associated with blepharitis, and in vivo rabbit eye tolerability of a cyclodextrin-complexed methylglyoxal (MGO) Manuka Honey microemulsion (MHME).In vitro phase: Bacterial growth inhibition was assessed by area under the growth curve (AUC) for Staphylococcus aureus, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for S. aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa with cyclodextrin-complexed and uncomplexed Manuka honey were determined. In vivo phase: Six rabbits were administered 20 µL of MHME (at 1:10 dilution) to the right eye (treated) and 20 µL of saline to the left eye (control) daily, for 5 days. Tear evaporation, production, osmolarity, lipid layer, conjunctival hyperaemia and fluorescein staining were assessed daily, before and 15 min after instillation.In vitro phase: The relative AUC for cyclodextrin-complexed Manuka honey was lower than that of uncomplexed honey at both 250 and 550 mg/kg of MGO (both p <0.05). Cyclodextrin-complexed honey had lower MIC and MBC than uncomplexed honey for both S. aureus and S. epidermidis, but not P. aeruginosa. In vivo phase: No significant changes were observed in the parameters assessed in either treated or control eyes (all p >0.05).Overall, antimicrobial potency of cyclodextrin-complexed Manuka honey was greater than uncomplexed honey. No significant immediate or cumulative adverse effects were observed with MHME application on rabbit eyes, supporting future conduct of clinical safety and tolerability trials in human subjects. en
dc.format.medium Electronic-eCollection en
dc.language eng en
dc.relation.ispartofseries BMJ open ophthalmology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by-nc/4.0/ en
dc.title Preclinical development of MGO Manuka Honey microemulsion for blepharitis management. en
dc.type Journal Article en
dc.identifier.doi 10.1136/bmjophth-2016-000065 en
pubs.issue 1 en
pubs.begin-page e000065 en
pubs.volume 1 en
dc.rights.holder Copyright: The authors en
dc.identifier.pmid 29354709 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype other en
pubs.subtype Journal Article en
pubs.elements-id 709617 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id School of Medicine en
pubs.org-id Ophthalmology Department en
dc.identifier.eissn 2397-3269 en
pubs.record-created-at-source-date 2018-01-23 en
pubs.dimensions-id 29354709 en


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