Massive parallel sequencing of solid tumours - challenges and opportunities for pathologists.

Show simple item record Harris, Gavin en O'Toole, Sandra en George, Peter en Browett, Peter en Print, Cristin en 2018-11-21T02:42:25Z en 2017-01 en
dc.identifier.issn 0309-0167 en
dc.identifier.uri en
dc.description.abstract The role of pathologists is to provide diagnostic, prognostic and predictive data to enable clinical colleagues to manage patients optimally. Current histo/anatomical pathology is predominantly morphology-based, with the addition of biomarkers, applied largely through immunohistochemistry, fluorescence in-situ hybridization (FISH) and a limited range of polymerase chain reaction (PCR)-based molecular tests. The desire to apply genomics to the clinical care of patients has been facilitated by the human genome project and subsequently by high-throughput technologies known collectively as massive parallel sequencing (MPS, also referred to as next-generation sequencing, NGS). The use of MPS to identify mutations/variants and tissue RNA expression profiles for diagnosis, prognostication and targeted therapy stratification is now a reality in many clinical specialities. If histopathologists are considered experts in solid tumour pathology, MPS potentially falls within their scope; however, it challenges our predominant morphology-based paradigm. This review summarizes and comments on the current and future state of play of MPS for the practising histopathologist. It will focus on somatic mutations in solid tumours and will challenge histopathologists to take further leadership roles in this area. en
dc.format.medium Print en
dc.language eng en
dc.relation.ispartofseries Histopathology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Humans en
dc.subject Neoplasms en
dc.subject Pathology en
dc.subject High-Throughput Nucleotide Sequencing en
dc.subject Pathologists en
dc.title Massive parallel sequencing of solid tumours - challenges and opportunities for pathologists. en
dc.type Journal Article en
dc.identifier.doi 10.1111/his.13067 en
pubs.issue 1 en
pubs.begin-page 123 en
pubs.volume 70 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 27556576 en
pubs.end-page 133 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Review en
pubs.subtype Journal Article en
pubs.elements-id 554318 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1365-2559 en
pubs.record-created-at-source-date 2016-08-24 en
pubs.dimensions-id 27556576 en

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