Conducting electrospun fibres with polyanionic grafts as highly selective, label-free, electrochemical biosensor with a low detection limit for non-Hodgkin lymphoma gene.

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dc.contributor.author Kerr-Phillips, Thomas E en
dc.contributor.author Aydemir, Nihan en
dc.contributor.author Chan, Wai en
dc.contributor.author Barker, David en
dc.contributor.author Malmstrom Pendred, Jenny en
dc.contributor.author Plesse, Cedric en
dc.contributor.author Travas-Sejdic, Jadranka en
dc.date.accessioned 2018-11-27T01:49:52Z en
dc.date.issued 2018-02 en
dc.identifier.issn 0956-5663 en
dc.identifier.uri http://hdl.handle.net/2292/44627 en
dc.description.abstract A highly selective, label-free sensor for the non-Hodgkin lymphoma gene, with an aM detection limit, utilizing electrochemical impedance spectroscopy (EIS) is presented. The sensor consists of a conducting electrospun fibre mat, surface-grafted with poly(acrylic acid) (PAA) brushes and a conducting polymer sensing element with covalently attached oligonucleotide probes. The sensor was fabricated from electrospun NBR rubber, embedded with poly(3,4-ethylenedioxythiophene) (PEDOT), followed by grafting poly(acrylic acid) brushes and then electrochemically polymerizing a conducting polymer monomer with ssDNA probe sequence pre-attached. The resulting non-Hodgkin lymphoma gene sensor showed a detection limit of 1aM (1 × 10-18mol/L), more than 400 folds lower compared to a thin-film analogue. The sensor presented extraordinary selectivity, with only 1%, 2.7% and 4.6% of the signal recorded for the fully non-complimentary, T-A and G-C base mismatch oligonucleotide sequences, respectively. We suggest that such greatly enhanced selectivity is due to the presence of negatively charged carboxylic acid moieties from PAA grafts that electrostatically repel the non-complementary and mismatch DNA sequences, overcoming the non-specific binding. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Biosensors & bioelectronics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Humans en
dc.subject Lymphoma, Non-Hodgkin en
dc.subject Polymers en
dc.subject Acrylic Resins en
dc.subject DNA en
dc.subject Biosensing Techniques en
dc.subject Base Pair Mismatch en
dc.subject Porosity en
dc.subject Bridged Bicyclo Compounds, Heterocyclic en
dc.title Conducting electrospun fibres with polyanionic grafts as highly selective, label-free, electrochemical biosensor with a low detection limit for non-Hodgkin lymphoma gene. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.bios.2017.09.042 en
pubs.begin-page 549 en
pubs.volume 100 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 29017070 en
pubs.end-page 555 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.elements-id 692465 en
pubs.org-id Engineering en
pubs.org-id Chemical and Materials Eng en
pubs.org-id Science en
pubs.org-id Chemistry en
dc.identifier.eissn 1873-4235 en
pubs.record-created-at-source-date 2017-10-11 en
pubs.dimensions-id 29017070 en


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