dc.contributor.author |
Gehlen, Lutz R |
en |
dc.contributor.author |
Gruenert, Gerd |
en |
dc.contributor.author |
Jones, M Beatrix |
en |
dc.contributor.author |
Rodley, Chris D |
en |
dc.contributor.author |
Langowski, Jörg |
en |
dc.contributor.author |
O'Sullivan, Justin |
en |
dc.date.accessioned |
2018-11-28T21:41:46Z |
en |
dc.date.issued |
2012-07 |
en |
dc.identifier.issn |
1949-1034 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/44664 |
en |
dc.description.abstract |
In recent years there has been considerable and growing interest in the 3-dimensional organization of genomes. In this manuscript we present an integrated computational-molecular study that produces an ensemble of high-resolution 3-dimensional conformations of the budding yeast genome. The compaction, folding and spatial organization of the chromosomes was based on empirical data determined using proximity-based ligation. Our models incorporate external constraints that allow the separation of gross organizational effects from those due to local interactions. Our models show that yeast chromosomes have preferred yet non-exclusive positions. They also identify interaction dependent clustering of tRNAs, early firing origins of replication, and Gal4 protein binding sites, yet the cluster composition is dynamic. Our results support a link between structure and transcription that occurs within the context of a flexible genome organization. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Nucleus (Austin, Tex.) |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Chromosomes, Fungal |
en |
dc.subject |
Saccharomyces cerevisiae |
en |
dc.subject |
Schizosaccharomyces |
en |
dc.subject |
RNA, Transfer |
en |
dc.subject |
Chromosome Positioning |
en |
dc.subject |
Nucleic Acid Conformation |
en |
dc.subject |
Genes, Fungal |
en |
dc.subject |
Genome, Fungal |
en |
dc.subject |
Algorithms |
en |
dc.subject |
Models, Molecular |
en |
dc.subject |
Chromosomal Position Effects |
en |
dc.subject |
Molecular Dynamics Simulation |
en |
dc.subject |
Genetic Loci |
en |
dc.title |
Chromosome positioning and the clustering of functionally related loci in yeast is driven by chromosomal interactions. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.4161/nucl.20971 |
en |
pubs.issue |
4 |
en |
pubs.begin-page |
370 |
en |
pubs.volume |
3 |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
22688649 |
en |
pubs.end-page |
383 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
364167 |
en |
pubs.org-id |
Liggins Institute |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Statistics |
en |
dc.identifier.eissn |
1949-1042 |
en |
pubs.record-created-at-source-date |
2012-09-27 |
en |
pubs.dimensions-id |
22688649 |
en |