dc.contributor.author |
Mugisho, Odunayo |
en |
dc.contributor.author |
Green, Colin |
en |
dc.contributor.author |
Zhang, Jie |
en |
dc.contributor.author |
Binz, Nicolette |
en |
dc.contributor.author |
Acosta Etchebarne, Monica |
en |
dc.contributor.author |
Rakoczy, Elizabeth |
en |
dc.contributor.author |
Rupenthal, Ilva |
en |
dc.date.accessioned |
2018-12-03T03:37:39Z |
en |
dc.date.issued |
2017-11-29 |
en |
dc.identifier.issn |
1422-0067 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/44802 |
en |
dc.description.abstract |
Diabetic retinopathy (DR) develops due to hyperglycemia and inflammation-induced vascular disruptions in the retina with connexin43 expression patterns in the disease still debated. Here, the effects of hyperglycemia and inflammation on connexin43 expression in vitro in a mouse model of DR and in human donor tissues were evaluated. Primary human retinal microvascular endothelial cells (hRMECs) were exposed to high glucose (HG; 25 mM) or pro-inflammatory cytokines IL-1β and TNF-α (10 ng/mL each) or both before assessing connexin43 expression. Additionally, connexin43, glial fibrillary acidic protein (GFAP), and plasmalemma vesicular associated protein (PLVAP) were labeled in wild-type (C57BL/6), Akita (diabetic), and Akimba (DR) mouse retinas. Finally, connexin43 and GFAP expression in donor retinas with confirmed DR was compared to age-matched controls. Co-application of HG and cytokines increased connexin43 expression in hRMECs in line with results seen in mice, with no significant difference in connexin43 or GFAP expression in Akita but higher expression in Akimba compared to wild-type mice. On PLVAP-positive vessels, connexin43 was higher in Akimba but unchanged in Akita compared to wild-type mice. Connexin43 expression appeared higher in donor retinas with confirmed DR compared to age-matched controls, similar to the distribution seen in Akimba mice and correlating with the in vitro results. Although connexin43 expression seems reduced in diabetes, hyperglycemia and inflammation present in the pathology of DR seem to increase connexin43 expression, suggesting a causal role of connexin43 channels in the disease progression. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
International journal of molecular sciences |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1661-6596/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Retina |
en |
dc.subject |
Animals |
en |
dc.subject |
Mice, Inbred C57BL |
en |
dc.subject |
Humans |
en |
dc.subject |
Mice |
en |
dc.subject |
Diabetic Retinopathy |
en |
dc.subject |
Hyperglycemia |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Inflammation |
en |
dc.subject |
Glial Fibrillary Acidic Protein |
en |
dc.subject |
Tumor Necrosis Factor-alpha |
en |
dc.subject |
Connexin 43 |
en |
dc.subject |
Interleukin-1beta |
en |
dc.title |
Immunohistochemical Characterization of Connexin43 Expression in a Mouse Model of Diabetic Retinopathy and in Human Donor Retinas. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.3390/ijms18122567 |
en |
pubs.issue |
12 |
en |
pubs.volume |
18 |
en |
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
29186067 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
718539 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Optometry and Vision Science |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Ophthalmology Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1422-0067 |
en |
pubs.record-created-at-source-date |
2017-11-30 |
en |
pubs.dimensions-id |
29186067 |
en |