dc.contributor.author |
Soh, Kar Yan |
en |
dc.contributor.author |
Hughes, Jacelyn |
en |
dc.contributor.author |
Proft, Thomas |
en |
dc.date.accessioned |
2018-12-03T04:02:21Z |
en |
dc.date.issued |
2018-08 |
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dc.identifier.citation |
Journal of biochemistry 164(2):165-171 Aug 2018 |
en |
dc.identifier.issn |
0021-924X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/44821 |
en |
dc.description.abstract |
Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5' nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. In vitro studies have shown that SpnA is a nuclease that cleaves ssDNA and dsDNA, including the DNA backbone of neutrophil extracellular traps. S5nA was shown to hydrolyse AMP and ADP, but not ATP, to generate the immunomodulatory molecule adenosine. S5nA also generates the macrophage-toxic deoxyadenosine from dAMP. However, detailed in vivo studies of the two enzymes have been hampered by difficulties with using current animal models for this exclusive human pathogen. Here we report the identification of two novel enzymes from the fish pathogen Streptococcus iniae that show similarities to SpnA and S5nA in amino acid sequence, protein domain structure and biochemical properties. We propose that SpnAi and S5nAi are orthologues of the S. pyogenes enzymes, providing a rationale to analyse the in vivo function of the two enzymes using a S. iniae-zebrafish infection model. |
en |
dc.format.medium |
Print |
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dc.language |
eng |
en |
dc.relation.ispartofseries |
Journal of biochemistry |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights |
This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of biochemistry following peer review. The version of record Journal of biochemistry 164(2):165-171 Aug 2018 is available online at: http://dx.doi.org/10.1093/jb/mvy039 |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://academic.oup.com/journals/pages/access_purchase/rights_and_permissions/self_archiving_policy_b |
en |
dc.subject |
Animals |
en |
dc.subject |
Zebrafish |
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dc.subject |
Streptococcus pyogenes |
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dc.subject |
Streptococcal Infections |
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dc.subject |
Disease Models, Animal |
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dc.subject |
Deoxyribonucleases |
en |
dc.subject |
5'-Nucleotidase |
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dc.subject |
Amino Acid Sequence |
en |
dc.subject |
Protein Conformation |
en |
dc.subject |
Streptococcus iniae |
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dc.title |
Orthologues of Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5'-nucleotidase A (S5nA) found in Streptococcus iniae. |
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dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1093/jb/mvy039 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
165 |
en |
pubs.volume |
164 |
en |
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
29659850 |
en |
pubs.end-page |
171 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
737876 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1756-2651 |
en |
pubs.record-created-at-source-date |
2018-04-17 |
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pubs.dimensions-id |
29659850 |
en |