How a decreased fibrillar interconnectivity influences stiffness and swelling properties during early cartilage degeneration.

Show simple item record Nickien, Mieke en Thambyah, Ashvin en Broom, Neil en 2018-12-05T20:42:58Z en 2017-11 en
dc.identifier.citation Journal of the Mechanical Behavior of Biomedical Materials 75:390-398 Nov 2017 en
dc.identifier.issn 1751-6161 en
dc.identifier.uri en
dc.description.abstract The functional coupling between the fibrillar network and the high-swelling proteoglycans largely determines the mechanical properties of the articular cartilage matrix. The objective of this new study was to show specifically how changes in fibrillar interconnectivity arising from early cartilage degeneration influence transverse stiffness and swelling properties at the tissue level.Radial zone transverse layers of cartilage matrix were obtained from intact and mildly degenerate bovine patellae. Each layer was then subdivided to assess tensile stiffness, free-swelling response, glycosaminoglycan (GAG) content, and micro- and ultra-structural features.The tensile modulus was significantly lower and the degree of swelling significantly higher for the degenerate matrix compared to the intact. Scanning electron microscopy revealed a homogeneous response to transverse strain in the intact cartilage, whereas large non-fibrillar spaces between fibril aggregates were visible in the degenerate matrix. Although there were no significant differences in GAG content it did correlate significantly with stiffness and swelling in the intact samples but not in the degenerate.The lower degree of fibril network interconnectivity in the degenerate matrix led to both a decreased transverse stiffness and reduced resistance to osmotic swelling. This network 'de-structuring' also resulted in a reduced functional interaction between the fibrillar network and the proteoglycans. The study provides new insights into the role of the fibrillar network and how changes in the network arising from the degenerative cascade will influence tissue level behaviour. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Journal of the mechanical behavior of biomedical materials en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.subject Cartilage, Articular en
dc.subject Patella en
dc.subject Extracellular Matrix en
dc.subject Animals en
dc.subject Cattle en
dc.subject Collagen en
dc.subject Glycosaminoglycans en
dc.subject Proteoglycans en
dc.subject Microscopy, Electron, Scanning en
dc.title How a decreased fibrillar interconnectivity influences stiffness and swelling properties during early cartilage degeneration. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.jmbbm.2017.07.042 en
pubs.begin-page 390 en
pubs.volume 75 en
dc.rights.holder Copyright: Elsevier Ltd en
dc.identifier.pmid 28803113 en
pubs.end-page 398 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 650550 en Engineering en Chemical and Materials Eng en
dc.identifier.eissn 1878-0180 en
pubs.record-created-at-source-date 2017-08-14 en
pubs.dimensions-id 28803113 en

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