Effect of silver nanoparticles on human primary keratinocytes.

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dc.contributor.author Szmyd, Radoslaw en
dc.contributor.author Goralczyk, Anna Grazyna en
dc.contributor.author Skalniak, Lukasz en
dc.contributor.author Cierniak, Agnieszka en
dc.contributor.author Lipert, Barbara en
dc.contributor.author Filon, Francesca Larese en
dc.contributor.author Crosera, Matteo en
dc.contributor.author Borowczyk, Julia en
dc.contributor.author Laczna, Eliza en
dc.contributor.author Drukala, Justyna en
dc.contributor.author Klein, Andrzej en
dc.contributor.author Jura, Jolanta en
dc.date.accessioned 2018-12-06T00:24:12Z en
dc.date.issued 2013-01 en
dc.identifier.citation Biological Chemistry 394(1):113-123 01 Jan 2013 en
dc.identifier.issn 1431-6730 en
dc.identifier.uri http://hdl.handle.net/2292/44881 en
dc.description.abstract Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are used extensively in a range of medical settings, particularly in wound dressings but also in cosmetics. This study was undertaken to examine the potential toxic effects of 15 nm polyvinylpyrrolidone-coated AgNPs on primary normal human epidermal keratinocytes (NHEK). Cells were treated with different concentrations of AgNPs and then cell viability, metabolic activity and other biological and biochemical aspects of keratinocytes functioning were studied. We observed that AgNPs decrease keratinocyte viability, metabolism and also proliferatory and migratory potential of these cells. Moreover, longer exposure resulted in activation of caspase 3/7 and DNA damage. Our studies show for the first time, that AgNPs may present possible danger for primary keratinocytes, concerning activation of genotoxic and cytotoxic processes depending on the concentration. en
dc.format.medium Print en
dc.language eng en
dc.relation.ispartofseries Biological chemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://www.degruyter.com/dg/page/repository-policy en
dc.subject Cells, Cultured en
dc.subject Keratinocytes en
dc.subject Humans en
dc.subject DNA Damage en
dc.subject Silver en
dc.subject Drug Evaluation, Preclinical en
dc.subject Cell Survival en
dc.subject Structure-Activity Relationship en
dc.subject Dose-Response Relationship, Drug en
dc.subject Caspase 3 en
dc.subject Caspase 7 en
dc.subject Metal Nanoparticles en
dc.title Effect of silver nanoparticles on human primary keratinocytes. en
dc.type Journal Article en
dc.identifier.doi 10.1515/hsz-2012-0202 en
pubs.issue 1 en
pubs.begin-page 113 en
pubs.volume 394 en
dc.rights.holder Copyright: Walter de Gruyter en
pubs.end-page 123 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 741949 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Auckland Cancer Research en
dc.identifier.eissn 1437-4315 en
pubs.record-created-at-source-date 2013-02-28 en
pubs.dimensions-id 23091270 en

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