Markers for human brain pericytes and smooth muscle cells.

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Show simple item record Smyth, Leon en Rustenhoven, Justin en Scotter, Emma en Schweder, Patrick en Faull, Richard en Park, In en Dragunow, Michael en 2018-12-10T02:24:59Z en 2018-10 en
dc.identifier.issn 0891-0618 en
dc.identifier.uri en
dc.description.abstract Brain pericytes and vascular smooth muscle cells (vSMCs) are a critical component of the neurovascular unit and are important in regulating cerebral blood flow and blood-brain barrier integrity. Identification of subtypes of mural cells in tissue and in vitro is important to any study of their function, therefore we identified distinct mural cell morphologies in neurologically normal post-mortem human brain. Further, the distribution of mural cell markers platelet-derived growth factor receptor-β (PDGFRβ), α-smooth muscle actin (αSMA), CD13, neural/glial antigen-2 (NG2), CD146 and desmin was examined. We determined that PDGFRβ, NG2, CD13, and CD146 were expressed in capillary-associated pericytes. NG2, and CD13 were also present on vSMCs in large vessels, however abundant CD146 and desmin staining was also detected in vSMCs on large vessels, co-labelling with αSMA. To determine whether cultures recapitulated observations from tissue, primary human brain pericytes derived from neurologically normal autopsies were analysed for the presence of pericyte markers by immunocytochemistry, western blotting and qPCR. The proteins observed in brain pericytes in tissue (PDGFRβ, αSMA, desmin, CD146, CD13, and NG2) were present in vitro, validating a panel of proteins that can be used to label brain pericytes and vSMCs in tissue and in vitro. Finally, we showed that the proteins CD146 and desmin that are expressed on large vessels in situ, are also selective markers of a smooth muscle cell phenotype in vitro. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Journal of chemical neuroanatomy en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Blood-Brain Barrier en
dc.subject Pericytes en
dc.subject Brain en
dc.subject Myocytes, Smooth Muscle en
dc.subject Humans en
dc.subject Desmin en
dc.subject Actins en
dc.subject Receptor, Platelet-Derived Growth Factor beta en
dc.subject Biomarkers en
dc.subject CD13 Antigens en
dc.subject CD146 Antigen en
dc.title Markers for human brain pericytes and smooth muscle cells. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.jchemneu.2018.06.001 en
pubs.begin-page 48 en
pubs.volume 92 en
dc.rights.holder Copyright: The author en
pubs.end-page 60 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 745387 en Medical and Health Sciences en Medical Sciences en Anatomy and Medical Imaging en Pharmacology en Science en Biological Sciences en
dc.identifier.eissn 1873-6300 en
pubs.record-created-at-source-date 2018-06-11 en
pubs.dimensions-id 29885791 en

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