dc.contributor.author |
Maradze, Diana |
en |
dc.contributor.author |
Musson, David |
en |
dc.contributor.author |
Zheng, Yufeng |
en |
dc.contributor.author |
Cornish, Jillian |
en |
dc.contributor.author |
Lewis, Mark |
en |
dc.contributor.author |
Liu, Yang |
en |
dc.date.accessioned |
2018-12-10T02:27:05Z |
en |
dc.date.issued |
2018-07-03 |
en |
dc.identifier.citation |
Scientific reports 8(1):10003 03 Jul 2018 |
en |
dc.identifier.issn |
2045-2322 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/44972 |
en |
dc.description.abstract |
The aim of this study was to gain an understanding on the collective cellular effects of magnesium (Mg) corrosion products on the behaviour of cells responsible for bone formation and remodelling. The response of mesenchymal stem cells (MSCs) and osteoclast cells to both soluble (Mg ions) and insoluble (granule) corrosion products were recapitulated in vitro by controlling the concentration of the corrosion products. Clearance of corrosion granules by MSCs was also inspected by TEM analysis at sub-cellular level. The effect of Mg corrosion products varied depending on the state of differentiation of cells, concentration and length of exposure. The presence of the corrosion products significantly altered the cells' metabolic and proliferative activities, which further affected cell fusion/differentiation. While cells tolerated higher than physiological range of Mg concentration (16 mM), concentrations below 10 mM were beneficial for cell growth. Furthermore, MSCs were shown to contribute to the clearance of intercellular corrosion granules, whilst high concentrations of corrosion products negatively impacted on osteoclast progenitor cell number and mature osteoclast cell function. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Scientific reports |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Osteoclasts |
en |
dc.subject |
Mesenchymal Stem Cells |
en |
dc.subject |
Animals |
en |
dc.subject |
Humans |
en |
dc.subject |
Mice |
en |
dc.subject |
Ions |
en |
dc.subject |
Magnesium |
en |
dc.subject |
Bone Remodeling |
en |
dc.subject |
Cell Communication |
en |
dc.subject |
Cell Differentiation |
en |
dc.subject |
Cell Proliferation |
en |
dc.subject |
Osteogenesis |
en |
dc.subject |
Corrosion |
en |
dc.subject |
RAW 264.7 Cells |
en |
dc.title |
High Magnesium Corrosion Rate has an Effect on Osteoclast and Mesenchymal Stem Cell Role During Bone Remodelling. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1038/s41598-018-28476-w |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
10003 |
en |
pubs.volume |
8 |
en |
dc.rights.holder |
Copyright: The authors |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
747224 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
2045-2322 |
en |
pubs.record-created-at-source-date |
2018-07-04 |
en |
pubs.dimensions-id |
29968794 |
en |