Assembly of Protein Stacks With in Situ Synthesized Nanoparticle Cargo.

Show simple item record Manuguri, Sesha en Webster, Kyle en Yewdall, N Amy en An, Yiran en Venugopal, Hari en Bhugra, Vaibhav en Turner, Adrian en Domigan, Laura en Gerrard, Juliet en Williams, David en Malmstrom Pendred, Jenny en 2018-12-10T20:21:11Z en 2018-08 en
dc.identifier.issn 1530-6984 en
dc.identifier.uri en
dc.description.abstract The ability of proteins to form hierarchical structures through self-assembly provides an opportunity to synthesize and organize nanoparticles. Ordered nanoparticle assemblies are a subject of widespread interest due to the potential to harness their emergent functions. In this work, the toroidal-shaped form of the protein peroxiredoxin, which has a pore size of 7 nm, was used to organize iron oxyhydroxide nanoparticles. Iron in the form of Fe2+ was sequestered into the central cavity of the toroid ring using metal-binding sites engineered there and then hydrolyzed to form iron oxyhydroxide particles bound into the protein pore. By precise manipulation of the pH, the mineralized toroids were organized into stacks confining one-dimensional nanoparticle assemblies. We report the formation and the procedures leading to the formation of such nanostructures and their characterization by chromatography and microscopy. Electrostatic force microscopy clearly revealed the formation of iron-containing nanorods as a result of the self-assembly of the iron-loaded protein. This research bodes well for the use of peroxiredoxin as a template with which to form nanowires and structures for electronic and magnetic applications. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Nano letters en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Iron en
dc.subject Ferric Compounds en
dc.subject Protein Binding en
dc.subject Hydrogen-Ion Concentration en
dc.subject Particle Size en
dc.subject Nanotechnology en
dc.subject Porosity en
dc.subject Nanoparticles en
dc.subject Peroxiredoxins en
dc.subject Protein Multimerization en
dc.subject Static Electricity en
dc.title Assembly of Protein Stacks With in Situ Synthesized Nanoparticle Cargo. en
dc.type Journal Article en
dc.identifier.doi 10.1021/acs.nanolett.8b02055 en
pubs.issue 8 en
pubs.begin-page 5138 en
pubs.volume 18 en
dc.rights.holder Copyright: The author en
pubs.end-page 5145 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 750815 en Engineering en Chemical and Materials Eng en Science en Biological Sciences en Chemistry en
dc.identifier.eissn 1530-6992 en
pubs.record-created-at-source-date 2018-07-27 en
pubs.dimensions-id 30047268 en

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