Screening of Cytotoxicity and Anti-Inflammatory Properties of Feijoa Extracts Using Genetically Modified Cell Models Targeting TLR2, TLR4 and NOD2 Pathways, and the Implication for Inflammatory Bowel Disease.

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dc.contributor.author Peng, Yaoyao en
dc.contributor.author Bishop, Karen en
dc.contributor.author Ferguson, Lynnette en
dc.contributor.author Quek, Siew-Young en
dc.date.accessioned 2019-03-08T02:31:54Z en
dc.date.issued 2018-08-31 en
dc.identifier.citation Nutrients 10(9) 31 Aug 2018 en
dc.identifier.issn 2072-6643 en
dc.identifier.uri http://hdl.handle.net/2292/45846 en
dc.description.abstract Feijoa has been increasingly studied in the recent decade, while investigations into its bioactivities including anti-inflammatory activity are lacking. In this article, the cytotoxicity and anti-inflammatory properties of feijoa extracts, from flesh, peel and whole fruit, from four cultivars namely APOLLO, UNIQUE, OPAL STAR and WIKI TU are presented. Three inflammatory pathways, Toll-like receptor 2 (TLR2), TLR4 and nucleotide-binding oligomerization domain-containing protein 2 (NOD2), were investigated using genetically modified cell models namely HEK-Blue™ hTLR2, HEK-Blue™ hTLR4, NOD2-WT and NOD2-G908R. Results show that feijoa peel extract induced higher cytotoxicity than flesh and whole fruit extracts, and the APOLLO cultivar was the most anti-inflammatory among the four tested cultivars. The anti-inflammatory activity of feijoa flesh was detected only through the TLR2 pathway, and the activity of feijoa peel and whole fruit was evident mainly through the TLR2 and NOD2 pathways. Most notably, feijoa anti-inflammatory activity was superior to ibuprofen particularly through the TLR2 pathway, with significantly lower secreted embryonic alkaline phosphatase IC50 concentrations (7.88, 12.81, 30.84 and 442.90 μg/mL for APOLLO flesh, peel, whole fruit extract and ibuprofen respectively). These findings indicate that feijoa has great potential to be used in the treatment and prevention of inflammation-related diseases including inflammatory bowel disease. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Nutrients en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Cell Line en
dc.subject Humans en
dc.subject Feijoa en
dc.subject Inflammatory Bowel Diseases en
dc.subject Anti-Inflammatory Agents en
dc.subject Plant Extracts en
dc.subject Phytotherapy en
dc.subject Signal Transduction en
dc.subject Toll-Like Receptor 2 en
dc.subject Toll-Like Receptor 4 en
dc.subject Nod2 Signaling Adaptor Protein en
dc.title Screening of Cytotoxicity and Anti-Inflammatory Properties of Feijoa Extracts Using Genetically Modified Cell Models Targeting TLR2, TLR4 and NOD2 Pathways, and the Implication for Inflammatory Bowel Disease. en
dc.type Journal Article en
dc.identifier.doi 10.3390/nu10091188 en
pubs.issue 9 en
pubs.volume 10 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 753121 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Auckland Cancer Research en
pubs.org-id Science en
pubs.org-id Chemistry en
dc.identifier.eissn 2072-6643 en
pubs.record-created-at-source-date 2018-09-12 en
pubs.dimensions-id 30200338 en


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