Profiling of headspace volatiles from Escherichia coli cultures using silicone-based sorptive media and thermal desorption GC-MS.

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dc.contributor.author Devaraj, Harish en
dc.contributor.author Pook, Chris en
dc.contributor.author Swift, Simon en
dc.contributor.author Aw, Kean en
dc.contributor.author McDaid, Andrew en
dc.date.accessioned 2019-03-11T20:50:15Z en
dc.date.issued 2018-11-05 en
dc.identifier.issn 1615-9306 en
dc.identifier.uri http://hdl.handle.net/2292/45915 en
dc.description.abstract Headspace sorptive extraction technique using silicone based sorptive media coated stir bars is used for the first time here to extract, identify, and quantify heavy volatile organic compounds present in Escherichia coli culture headspace. Detection of infection presence is largely accomplished in laboratories through physical sampling and subsequent growth of cultures for biochemical testing. The use of volatile biomarkers released from pathogens as indicators for pathogenic presence can vastly reduce the time needed whilst improving the success rates for infection detection. To validate this, by using a contactless headspace sorptive extraction technique, the volatile compounds released from E. coli, grown in vitro, have been extracted and identified. Two different sorptive media for extracting these headspace volatiles were compared in this study and the identified volatiles were quantified. The large phase volume and wider retention of this sorptive technique compared to traditional sampling approach enabled preconcentration and collection of wider range of volatiles towards developing an extensive database of such heavy volatiles associated with E. coli. This supplements the existing data of potential bacterial markers and use of internal standards in these tests allows semi-quantitative estimation of these compounds towards the development and optimization of novel pathogen sensing devices. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Journal of separation science en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Escherichia coli en
dc.subject Silicones en
dc.subject Temperature en
dc.subject Molecular Structure en
dc.subject Adsorption en
dc.subject Particle Size en
dc.subject Surface Properties en
dc.subject Gas Chromatography-Mass Spectrometry en
dc.subject Volatile Organic Compounds en
dc.title Profiling of headspace volatiles from Escherichia coli cultures using silicone-based sorptive media and thermal desorption GC-MS. en
dc.type Journal Article en
dc.identifier.doi 10.1002/jssc.201800684 en
pubs.issue 22 en
pubs.begin-page 4133 en
pubs.volume 41 en
dc.rights.holder Copyright: The author en
pubs.end-page 4141 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.elements-id 752558 en
pubs.org-id Engineering en
pubs.org-id Mechanical Engineering en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
dc.identifier.eissn 1615-9314 en
pubs.record-created-at-source-date 2018-08-30 en
pubs.dimensions-id 30156752 en


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