dc.contributor.author |
Hong, Jiwon |
en |
dc.contributor.author |
Nachkebia, Shorena |
en |
dc.contributor.author |
Tun, Soe Min |
en |
dc.contributor.author |
Petzer, Amorita |
en |
dc.contributor.author |
Windsor, John |
en |
dc.contributor.author |
Hickey, Anthony |
en |
dc.contributor.author |
Phillips, Anthony |
en |
dc.date.accessioned |
2019-03-20T03:56:41Z |
en |
dc.date.issued |
2018-12 |
en |
dc.identifier.issn |
0163-2116 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/46168 |
en |
dc.description.abstract |
BACKGROUND:Triglyceride-rich lipoproteins are important in dietary lipid absorption and subsequent energy distribution in the body. Their importance in the gut-lymph may have been overlooked in sepsis, the most common cause of critical illness, and in gut ischemia-reperfusion injury, a common feature of many critical illnesses. AIMS:We aimed to undertake an exploratory study of triglyceride-rich lipoprotein fractions in gut-lymph using untargeted metabolic profiling to identify altered metabolites in sepsis or gut ischemia-reperfusion. METHODS:The gut-lymph was collected from rodent sham, sepsis, and gut ischemia-reperfusion models. The triglyceride-rich lipoprotein-enriched fractions isolated from the gut-lymph were subjected to a dual metabolomics analysis approach: non-polar metabolite analysis by ultra-high performance liquid chromatography-mass spectrometry and polar metabolite analysis by gas chromatography-mass spectrometry. RESULTS:The metabolite analysis of gut-lymph triglyceride-rich lipoprotein fractions revealed a significant increase (FDR-adjusted P value < 0.05) in myo-inositol in the sepsis group and monoacylglycerols [(18:1) and (18:2)] in gut ischemia-reperfusion. There were no significantly increased specific metabolites in the lipoprotein-enriched fractions of both sepsis and gut ischemia-reperfusion. In contrast, there was a widespread decrease in multiple lipid species in sepsis (35 out of 190; adjusted P < 0.05), but not in the gut ischemia-reperfusion. CONCLUSIONS:Increased levels of myo-inositol and monoacylglycerols, and decreased multiple lipid species in the gut-lymph triglyceride-rich lipoprotein fraction could be candidates for new biomarkers and/or involved in the progression of sepsis and gut ischemia-reperfusion pathobiology. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Digestive diseases and sciences |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Chyle |
en |
dc.subject |
Animals |
en |
dc.subject |
Rats |
en |
dc.subject |
Rats, Sprague-Dawley |
en |
dc.subject |
Sepsis |
en |
dc.subject |
Reperfusion Injury |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Inositol |
en |
dc.subject |
Triglycerides |
en |
dc.subject |
Lipoproteins |
en |
dc.subject |
Chromatography, Liquid |
en |
dc.subject |
Monoglycerides |
en |
dc.subject |
Mass Spectrometry |
en |
dc.subject |
Metabolomics |
en |
dc.subject |
Gastrointestinal Absorption |
en |
dc.subject |
Biomarkers |
en |
dc.title |
Altered Metabolic Profile of Triglyceride-Rich Lipoproteins in Gut-Lymph of Rodent Models of Sepsis and Gut Ischemia-Reperfusion Injury. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s10620-018-5270-6 |
en |
pubs.issue |
12 |
en |
pubs.begin-page |
3317 |
en |
pubs.volume |
63 |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
3328 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
753130 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Surgery Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1573-2568 |
en |
pubs.record-created-at-source-date |
2018-09-06 |
en |
pubs.dimensions-id |
30182310 |
en |