Altered Metabolic Profile of Triglyceride-Rich Lipoproteins in Gut-Lymph of Rodent Models of Sepsis and Gut Ischemia-Reperfusion Injury.

Show simple item record Hong, Jiwon en Nachkebia, Shorena en Tun, Soe Min en Petzer, Amorita en Windsor, John en Hickey, Anthony en Phillips, Anthony en 2019-03-20T03:56:41Z en 2018-12 en
dc.identifier.issn 0163-2116 en
dc.identifier.uri en
dc.description.abstract BACKGROUND:Triglyceride-rich lipoproteins are important in dietary lipid absorption and subsequent energy distribution in the body. Their importance in the gut-lymph may have been overlooked in sepsis, the most common cause of critical illness, and in gut ischemia-reperfusion injury, a common feature of many critical illnesses. AIMS:We aimed to undertake an exploratory study of triglyceride-rich lipoprotein fractions in gut-lymph using untargeted metabolic profiling to identify altered metabolites in sepsis or gut ischemia-reperfusion. METHODS:The gut-lymph was collected from rodent sham, sepsis, and gut ischemia-reperfusion models. The triglyceride-rich lipoprotein-enriched fractions isolated from the gut-lymph were subjected to a dual metabolomics analysis approach: non-polar metabolite analysis by ultra-high performance liquid chromatography-mass spectrometry and polar metabolite analysis by gas chromatography-mass spectrometry. RESULTS:The metabolite analysis of gut-lymph triglyceride-rich lipoprotein fractions revealed a significant increase (FDR-adjusted P value < 0.05) in myo-inositol in the sepsis group and monoacylglycerols [(18:1) and (18:2)] in gut ischemia-reperfusion. There were no significantly increased specific metabolites in the lipoprotein-enriched fractions of both sepsis and gut ischemia-reperfusion. In contrast, there was a widespread decrease in multiple lipid species in sepsis (35 out of 190; adjusted P < 0.05), but not in the gut ischemia-reperfusion. CONCLUSIONS:Increased levels of myo-inositol and monoacylglycerols, and decreased multiple lipid species in the gut-lymph triglyceride-rich lipoprotein fraction could be candidates for new biomarkers and/or involved in the progression of sepsis and gut ischemia-reperfusion pathobiology. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Digestive diseases and sciences en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Chyle en
dc.subject Animals en
dc.subject Rats en
dc.subject Rats, Sprague-Dawley en
dc.subject Sepsis en
dc.subject Reperfusion Injury en
dc.subject Disease Models, Animal en
dc.subject Inositol en
dc.subject Triglycerides en
dc.subject Lipoproteins en
dc.subject Chromatography, Liquid en
dc.subject Monoglycerides en
dc.subject Mass Spectrometry en
dc.subject Metabolomics en
dc.subject Gastrointestinal Absorption en
dc.subject Biomarkers en
dc.title Altered Metabolic Profile of Triglyceride-Rich Lipoproteins in Gut-Lymph of Rodent Models of Sepsis and Gut Ischemia-Reperfusion Injury. en
dc.type Journal Article en
dc.identifier.doi 10.1007/s10620-018-5270-6 en
pubs.issue 12 en
pubs.begin-page 3317 en
pubs.volume 63 en
dc.rights.holder Copyright: The author en
pubs.end-page 3328 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 753130 en Medical and Health Sciences en School of Medicine en Surgery Department en Science en Biological Sciences en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1573-2568 en
pubs.record-created-at-source-date 2018-09-06 en
pubs.dimensions-id 30182310 en

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