Macrophages enhance Vegfa-driven angiogenesis in an embryonic zebrafish tumour xenograft model.

Show simple item record Britto, DD en Wyroba, B en Chen, Wenxuan en Lockwood, RA en Tran, Bao en Shepherd, PR en Hall, Christopher en Crosier, Kathryn en Crosier, Philip en Astin, Jonathan en 2019-03-20T03:56:49Z en 2018-11-05 en
dc.identifier.citation Disease models & mechanisms 11(12) 29 Nov 2018 en
dc.identifier.issn 1754-8403 en
dc.identifier.uri en
dc.description.abstract Tumour angiogenesis has long been a focus of anti-cancer therapy, however, anti-angiogenic cancer treatment strategies have had limited clinical success. Tumour-associated myeloid cells are believed to play a role in the resistance of cancer towards anti-angiogenesis therapy, but the mechanisms by which they do this are unclear. A zebrafish embryonic xenograft model has been developed to investigate the mechanisms of tumour angiogenesis and as an assay to screen anti-angiogenic compounds. In this study, we used cell ablation techniques to remove either macrophages or neutrophils and assessed their contribution towards zebrafish xenograft angiogenesis by quantitating levels of graft vascularisation. The ablation of macrophages, but not neutrophils, caused a strong reduction in tumour xenograft vascularisation and time-lapse imaging demonstrated that tumour xenograft macrophages directly associated with the migrating tip of developing tumour blood vessels. Finally, we found that while macrophages are required for vascularisation in xenografts that either secrete VEGFA or overexpress zebrafish vegfaa, they are not required for the vascularisation of grafts with low levels of VEGFA, suggesting that zebrafish macrophages can enhance Vegfa-driven tumour angiogenesis. The importance of macrophages to this angiogenic response suggests that this model could be used to further investigate the interplay between myeloid cells and tumour vascularisation. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.publisher Company of Biologists en
dc.relation.ispartofseries Disease Models & Mechanisms en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.title Macrophages enhance Vegfa-driven angiogenesis in an embryonic zebrafish tumour xenograft model. en
dc.type Journal Article en
dc.identifier.doi 10.1242/dmm.035998 en
pubs.issue 12 en
pubs.volume 11 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 756096 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1754-8411 en
pubs.record-created-at-source-date 2018-11-20 en
pubs.dimensions-id 30396905 en

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