dc.contributor.advisor |
Tran, K |
en |
dc.contributor.advisor |
Taberner, A |
en |
dc.contributor.author |
Choi, Dong Hoon |
en |
dc.date.accessioned |
2019-05-23T02:18:45Z |
en |
dc.date.issued |
2019 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/46492 |
en |
dc.description.abstract |
Diabetes mellitus is a chronic disease arising from the inability of the body to produce or effectively use the hormone insulin. Diabetes is associated with an increased prevalence of heart failure, however the underlying reason is not yet clear. Mitochondrial dysfunction is thought to be the primary driver for the pathogenesis of diabetic heart failure through the accumulation of metabolites resulting in inhibition of cardiac contractility. In order to understand the effect of metabolite action on cardiac function, it is necessary to develop a device that is capable of measuring cardiac contractile mechanics in response to different metabolite concentrations. For this purpose, a new device for mechanical testing of chemically permeabilised cardiac tissues under a variety of chemical environments was developed and tested. The device comprised three subsystems: a mechanical perturbation system, a scanning microscope, and a multi-well tissue bath system. The device was calibrated and a series of tests were performed to characterise the measurement system. The capability of the device was exemplified through a series of experiments performed on cardiac trabeculae. The dynamic stiffness frequency response function was successfully measured in both intact and chemically permeabilised cardiac trabeculae. The characteristic shape of the measured dynamic stiffness frequency responses of chemically activated, permeabilised trabeculae were comparable to those observed in the literature. A mathematical model was fitted to an example of a measured frequency-response function, and the rate constants associated with the mechanical processes involved in cardiac contraction were obtained. |
en |
dc.publisher |
ResearchSpace@Auckland |
en |
dc.relation.ispartof |
Masters Thesis - University of Auckland |
en |
dc.relation.isreferencedby |
UoA99265160913702091 |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ |
en |
dc.title |
Mechanical Testing of Chemically Permeabilised Cardiac Tissues |
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dc.type |
Thesis |
en |
thesis.degree.discipline |
Bioengineering |
en |
thesis.degree.grantor |
The University of Auckland |
en |
thesis.degree.level |
Masters |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.elements-id |
773003 |
en |
pubs.org-id |
Bioengineering Institute |
en |
pubs.record-created-at-source-date |
2019-05-23 |
en |
dc.identifier.wikidata |
Q112948044 |
|