Cooking Your Data: challenging the use of orthodox SPME extraction temperatures in biological analysis

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Show simple item record McKenzie, EJ en Jayan, S en O'Sullivan, JM en
dc.coverage.spatial Auckland University of Technology, Auckland, New Zealand en 2019-05-28T03:42:51Z en 2019-01-30 en
dc.identifier.uri en
dc.description.abstract Solid-Phase Microextraction (SPME) is a widely used analytical technique for measuring volatile organic compounds in biosamples. The current literature on SPME recommends sample extraction temperature optimisation based primarily on obtaining a chromatographic profile with intense, narrow, well-separated peaks. However, studies in our laboratory comparing different extraction temperatures showed that increasing temperature significantly decreased the differences between the case-control study groups, and thus the relevance of the data to the research hypothesis. Contrary to recommendations, the least optimal chromatographic profile gave the most statistically significant results when looking at the differences between two groups. We speculate that the reliance on chromatographic characteristics has arisen for historical reasons. Advanced deconvolution software was developed late last century, yet the power of computer-based feature separation over chromatographic separation has not been fully exploited in some areas of applied mass spectrometry. Our study highlights the need for evidence-based rather than theory-based chromatographic method development. Keywords: SPME, Solid Phase MicroExtraction, GC-MS, Gas Chromatography-Mass Spectrometry, Deconvolution en
dc.relation.ispartof ANZSMS 27: Australian and New Zealand Society for Mass Spectrometry Conference 2019 en
dc.relation.ispartofseries ANZSMS27 27th Australian and New Zealand Society for Mass Spectrometry Conference, Conference Abstracts en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Cooking Your Data: challenging the use of orthodox SPME extraction temperatures in biological analysis en
dc.type Conference Item en
pubs.begin-page 30 en
dc.rights.holder Copyright: The author en en
pubs.end-page 30 en
pubs.finish-date 2019-02-03 en
pubs.start-date 2019-01-30 en
dc.rights.accessrights en
pubs.subtype Abstract en
pubs.elements-id 770297 en
pubs.record-created-at-source-date 2019-05-07 en

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