Rapid selection of an appropriate antibiotic

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dc.contributor.author Robertson, Julia en
dc.contributor.author Ou, Fang en
dc.contributor.author McGoverin, Cushla en
dc.contributor.author Vanholsbeeck, Frederique en
dc.contributor.author Swift, Simon en
dc.coverage.spatial Brisbane, Australia en
dc.date.accessioned 2019-05-28T04:34:55Z en
dc.date.issued 2018-11-20 en
dc.identifier.citation Australasian College for Infection Prevention and Control Conference 2018 (ACIPC 2018). 20 Nov 2018 en
dc.identifier.uri http://hdl.handle.net/2292/46734 en
dc.description.abstract Introduction Antibiotic resistance is a serious threat to public health. The empiric use of the wrong antibiotic occurs due to urgency in treatment combined with slow, culture-based diagnostic techniques. Inappropriate antibiotic choice can promote the development of antibiotic resistance. Methods We have developed a spectroscopic device (Optrode) to measure fluorescence from SYTO 9 and propidium iodide (PI) stained cells that can be used to enumerate the bacterial load. We propose a procedure using the Optrode that will take bacteria in a clinical sample, challenge with a panel of antibiotics and measure live/dead ratios to determine the best bactericidal choice. To this end, we have applied, and optimised, the methodology to detect live and dead E. coli in populations challenged with a range of antibiotics. We investigated the parameters required for lytic and non-lytic bactericidal antibiotics, and examined the influence of washing the sample before staining. Results We show that samples of antibiotic challenged culture do not require washing and that washing may cause loss of DNA from solution. Knockdown of E. coli treated with ampicillin and polymyxin B stained with SYTO 9 and PI can be detected by the Optrode in near real-time. For the non-lytic antibiotics, kanamycin and ciprofloxacin, the current analysis of live/dead staining was unable to detect the knockdown as indicated by plate counts. Conclusion The live/dead based Optrode assays can detect knockdown of E. coli challenged with lytic antibiotics in near real-time; however the assays are not very sensitive to the bactericidal action of non-lytic antibiotics. en
dc.description.uri http://www.acipcconference.com.au/4761 en
dc.relation.ispartof Australasian College for Infection Prevention and Control Conference 2018 (ACIPC 2018) en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Rapid selection of an appropriate antibiotic en
dc.type Conference Poster en
dc.rights.holder Copyright: The author en
pubs.author-url http://www.acipcconference.com.au/wp-content/uploads/2018/11/Julia-Robertson-Poster-Julia-Robertson.pdf en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.elements-id 761575 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id Science en
pubs.org-id Physics en
pubs.record-created-at-source-date 2019-02-15 en

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