dc.contributor.author |
Zhu, Mark |
en |
dc.contributor.author |
Tay, Mei Lin |
en |
dc.contributor.author |
Callon, Karen |
en |
dc.contributor.author |
Tuari, Donna |
en |
dc.contributor.author |
Zhao, Lei |
en |
dc.contributor.author |
Dray, Michael |
en |
dc.contributor.author |
Zhang, Jie |
en |
dc.contributor.author |
Dalbeth, Nicola |
en |
dc.contributor.author |
Munro, Jacob |
en |
dc.contributor.author |
Young, Simon |
en |
dc.contributor.author |
Coleman, Brendan |
en |
dc.contributor.author |
Patel, Dipika |
en |
dc.contributor.author |
Cornish, Jillian |
en |
dc.contributor.author |
Musson, David |
en |
dc.date.accessioned |
2019-05-28T21:20:48Z |
en |
dc.date.issued |
2019-05 |
en |
dc.identifier.issn |
1058-2746 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/46804 |
en |
dc.description.abstract |
BACKGROUND:Augmenting repairs with extracellular matrix-based scaffolds is a common option for rotator cuff tears. In this study, a new collagen scaffold was assessed for its efficacy in augmenting rotator cuff repair. METHODS:The collagen scaffold was assessed in vitro for cytocompatibility and retention of tenocyte phenotype using alamarBlue assays, fluorescent imaging, and real-time polymerase chain reaction. Immunogenicity was assessed in vitro by the activation of human monocytes. In vivo, by use of a modified rat rotator cuff defect model, supraspinatus tendon repairs were carried out in 40 animals. Overlay augmentation with the collagen scaffold was compared with unaugmented repairs. At 6 and 12 weeks postoperatively, the repairs were tested biomechanically to evaluate repair strength, as well as histologically to assess quality of healing. RESULTS:The collagen scaffold supported human tendon-derived cell growth in vitro, with cells demonstrating proliferation and appearing morphologically tenocytic over the experimental period. No immunogenic responses were provoked compared with suture material control. In vivo, augmentation with the scaffold improved the histologic scores at 12 weeks (8.4 of 15 vs 6.4 of 15, P = .032). However, no significant difference was detected with mechanical testing. CONCLUSION:The new collagen scaffold was supportive of cell growth in vitro and generated a minimal acute inflammatory response. In vivo, we observed an improvement in the histologic appearance of the repair at 12 weeks. However, a meaningful increase in biomechanical strength was not achieved. Further modification and improvement of the scaffold are required prior to consideration for clinical use. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Journal of shoulder and elbow surgery |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Extracellular Matrix |
en |
dc.subject |
Animals |
en |
dc.subject |
Rats |
en |
dc.subject |
Rats, Sprague-Dawley |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Collagen |
en |
dc.subject |
Wound Healing |
en |
dc.subject |
Tissue Scaffolds |
en |
dc.subject |
Biomechanical Phenomena |
en |
dc.subject |
Rotator Cuff Injuries |
en |
dc.title |
Overlay repair with a synthetic collagen scaffold improves the quality of healing in a rat rotator cuff repair model. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.jse.2018.11.044 |
en |
pubs.issue |
5 |
en |
pubs.begin-page |
949 |
en |
pubs.volume |
28 |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
958 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
761329 |
en |
pubs.org-id |
Academic Services |
en |
pubs.org-id |
Examinations |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.org-id |
Ophthalmology Department |
en |
pubs.org-id |
Surgery Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1532-6500 |
en |
pubs.record-created-at-source-date |
2019-02-07 |
en |
pubs.dimensions-id |
30723031 |
en |