Overlay repair with a synthetic collagen scaffold improves the quality of healing in a rat rotator cuff repair model.

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dc.contributor.author Zhu, Mark en
dc.contributor.author Tay, Mei Lin en
dc.contributor.author Callon, Karen en
dc.contributor.author Tuari, Donna en
dc.contributor.author Zhao, Lei en
dc.contributor.author Dray, Michael en
dc.contributor.author Zhang, Jie en
dc.contributor.author Dalbeth, Nicola en
dc.contributor.author Munro, Jacob en
dc.contributor.author Young, Simon en
dc.contributor.author Coleman, Brendan en
dc.contributor.author Patel, Dipika en
dc.contributor.author Cornish, Jillian en
dc.contributor.author Musson, David en
dc.date.accessioned 2019-05-28T21:20:48Z en
dc.date.issued 2019-05 en
dc.identifier.issn 1058-2746 en
dc.identifier.uri http://hdl.handle.net/2292/46804 en
dc.description.abstract BACKGROUND:Augmenting repairs with extracellular matrix-based scaffolds is a common option for rotator cuff tears. In this study, a new collagen scaffold was assessed for its efficacy in augmenting rotator cuff repair. METHODS:The collagen scaffold was assessed in vitro for cytocompatibility and retention of tenocyte phenotype using alamarBlue assays, fluorescent imaging, and real-time polymerase chain reaction. Immunogenicity was assessed in vitro by the activation of human monocytes. In vivo, by use of a modified rat rotator cuff defect model, supraspinatus tendon repairs were carried out in 40 animals. Overlay augmentation with the collagen scaffold was compared with unaugmented repairs. At 6 and 12 weeks postoperatively, the repairs were tested biomechanically to evaluate repair strength, as well as histologically to assess quality of healing. RESULTS:The collagen scaffold supported human tendon-derived cell growth in vitro, with cells demonstrating proliferation and appearing morphologically tenocytic over the experimental period. No immunogenic responses were provoked compared with suture material control. In vivo, augmentation with the scaffold improved the histologic scores at 12 weeks (8.4 of 15 vs 6.4 of 15, P = .032). However, no significant difference was detected with mechanical testing. CONCLUSION:The new collagen scaffold was supportive of cell growth in vitro and generated a minimal acute inflammatory response. In vivo, we observed an improvement in the histologic appearance of the repair at 12 weeks. However, a meaningful increase in biomechanical strength was not achieved. Further modification and improvement of the scaffold are required prior to consideration for clinical use. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Journal of shoulder and elbow surgery en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Extracellular Matrix en
dc.subject Animals en
dc.subject Rats en
dc.subject Rats, Sprague-Dawley en
dc.subject Disease Models, Animal en
dc.subject Collagen en
dc.subject Wound Healing en
dc.subject Tissue Scaffolds en
dc.subject Biomechanical Phenomena en
dc.subject Rotator Cuff Injuries en
dc.title Overlay repair with a synthetic collagen scaffold improves the quality of healing in a rat rotator cuff repair model. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.jse.2018.11.044 en
pubs.issue 5 en
pubs.begin-page 949 en
pubs.volume 28 en
dc.rights.holder Copyright: The author en
pubs.end-page 958 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.elements-id 761329 en
pubs.org-id Academic Services en
pubs.org-id Examinations en
pubs.org-id Medical and Health Sciences en
pubs.org-id School of Medicine en
pubs.org-id Medicine Department en
pubs.org-id Ophthalmology Department en
pubs.org-id Surgery Department en
pubs.org-id Science en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1532-6500 en
pubs.record-created-at-source-date 2019-02-07 en
pubs.dimensions-id 30723031 en

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