dc.contributor.author |
Wang, Weiguang |
en |
dc.contributor.author |
Junior, José Roberto Passarini |
en |
dc.contributor.author |
Nalesso, Paulo Roberto Lopes |
en |
dc.contributor.author |
Musson, David |
en |
dc.contributor.author |
Cornish, Jillian |
en |
dc.contributor.author |
Mendonça, Fernanda |
en |
dc.contributor.author |
Caetano, Guilherme Ferreira |
en |
dc.contributor.author |
Bártolo, Paulo |
en |
dc.date.accessioned |
2019-05-28T21:32:57Z |
en |
dc.date.issued |
2019-07 |
en |
dc.identifier.issn |
0928-4931 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/46825 |
en |
dc.description.abstract |
Scaffolds are important physical substrates for cell attachment, proliferation and differentiation. Multiple factors could influence the optimal design of scaffolds for a specific tissue, such as the geometry, the materials used to modulate cell proliferation and differentiation, its biodegradability and biocompatibility. The optimal design of a scaffold for a specific tissue strongly depends on both materials and manufacturing processes. Previous studies of human adipose-derived stem cells (hADSCs) seeded on poly(ε-caprolactone) (PCL)/graphene scaffolds have proved that the addition of small concentrations of graphene to PCL scaffolds improves cell proliferation. Based on such results, this paper further investigates, for the first time, both in vitro and in vivo characteristics of 3D printed PCL/graphene scaffolds. Scaffolds were evaluated from morphological, biological and short term immune response points of view. Results show that the produced scaffolds induce an acceptable level of immune response, suggesting high potential for in vivo applications. Finally, the scaffolds were used to treat a rat calvaria critical size defect with and without applying micro electrical stimulation (10 μA). Quantification of connective and new bone tissue formation and the levels of ALP, RANK, RANKL, OPG were considered. Results show that the use of scaffolds containing graphene and electrical stimulation seems to increase cell migration and cell influx, leading to new tissue formation, well-organized tissue deposition and bone remodelling. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Materials science & engineering. C, Materials for biological applications |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Bone and Bones |
en |
dc.subject |
Skull |
en |
dc.subject |
Cell Line |
en |
dc.subject |
Animals |
en |
dc.subject |
Humans |
en |
dc.subject |
Mice |
en |
dc.subject |
Rats, Wistar |
en |
dc.subject |
Graphite |
en |
dc.subject |
Alkaline Phosphatase |
en |
dc.subject |
Tissue Engineering |
en |
dc.subject |
Cell Proliferation |
en |
dc.subject |
Cell Survival |
en |
dc.subject |
Adsorption |
en |
dc.subject |
Male |
en |
dc.subject |
RANK Ligand |
en |
dc.subject |
Receptor Activator of Nuclear Factor-kappa B |
en |
dc.subject |
Osteoprotegerin |
en |
dc.subject |
Tissue Scaffolds |
en |
dc.subject |
Printing, Three-Dimensional |
en |
dc.title |
Engineered 3D printed poly(ɛ-caprolactone)/graphene scaffolds for bone tissue engineering. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.msec.2019.03.047 |
en |
pubs.begin-page |
759 |
en |
pubs.volume |
100 |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
770 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
766871 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1873-0191 |
en |
pubs.record-created-at-source-date |
2019-04-06 |
en |
pubs.dimensions-id |
30948113 |
en |