dc.contributor.author |
Thakur, Sachin |
en |
dc.contributor.author |
Chen, Ying-Shan |
en |
dc.contributor.author |
Houston, Zachary H |
en |
dc.contributor.author |
Fletcher, Nicholas |
en |
dc.contributor.author |
Barnett, Nigel L |
en |
dc.contributor.author |
Thurecht, Kristofer J |
en |
dc.contributor.author |
Rupenthal, Ilva |
en |
dc.contributor.author |
Parekh, Harendra S |
en |
dc.date.accessioned |
2019-06-13T02:45:11Z |
en |
dc.date.issued |
2019-03 |
en |
dc.identifier.issn |
0939-6411 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/47009 |
en |
dc.description.abstract |
The intravitreal route faces many challenges in rapidly and effectively reaching posterior eye pathology, with administered therapeutics experiencing non-specific distribution around and premature clearance from ocular tissues. Nanobubbles and ultrasound may improve outcomes of intravitreally administered drugs by influencing the directionality of drug-containing particle migration. In this study, we assessed the impact of trans-scleral or corneal ultrasound application on the distribution of intravitreally-injected nanobubbles. Rhodamine-tagged gas entrapped nanobubble formulations were prepared and injected into ex vivo bovine and porcine eyes and subjected to ultrasound (1 MHz, 0-2.5 W/cm2, 50-100% duty, 60 s). Bovine eyes were partially dissected to visualize the vitreous humor and particle migration was evaluated via optical fluorescence spectroscopy. Directional migration in porcine eyes was evaluated using a snap freezing protocol complemented by quantification of regional fluorescence. The impact on nanobubble migration following pars-plana injection and sequential ultrasound cycle application from scleral or corneal-surface positions was also assessed. Administration of ultrasound significantly enhanced the directional migration of nanobubbles in both ex vivo models, with multiple corneal ultrasound cycles promoting greater migration of dye-filled nanobubbles to posterior regions of the vitreous. Moreover, particles moved in a directional manner away from the ultrasound wave source demonstrating an ability to effectively control the rate and path of nanobubble migration. These findings establish an encouraging new and safe modality enabling rapid distribution of intravitreally-injected therapeutics where expeditious therapeutic intervention is warranted. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Vitreous Body |
en |
dc.subject |
Animals |
en |
dc.subject |
Cattle |
en |
dc.subject |
Swine |
en |
dc.subject |
Fluorescent Dyes |
en |
dc.subject |
Drug Delivery Systems |
en |
dc.subject |
Spectrometry, Fluorescence |
en |
dc.subject |
Nanocapsules |
en |
dc.subject |
Intravitreal Injections |
en |
dc.subject |
Ultrasonic Waves |
en |
dc.title |
Ultrasound-responsive nanobubbles for enhanced intravitreal drug migration: An ex vivo evaluation. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.ejpb.2019.01.014 |
en |
pubs.begin-page |
102 |
en |
pubs.volume |
136 |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
107 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
760307 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Pharmacy |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Ophthalmology Department |
en |
dc.identifier.eissn |
1873-3441 |
en |
pubs.record-created-at-source-date |
2019-01-21 |
en |
pubs.dimensions-id |
30660691 |
en |