dc.contributor.author |
Dharmaraj, Karthikeyan |
en |
dc.contributor.author |
Cui, Wei |
en |
dc.contributor.author |
Rikkerink, Erik HA |
en |
dc.contributor.author |
Templeton, Matthew |
en |
dc.date.accessioned |
2019-06-14T01:57:48Z |
en |
dc.date.issued |
2019-01-28 |
en |
dc.identifier.citation |
BMC research notes 12(1):63 28 Jan 2019 |
en |
dc.identifier.issn |
1756-0500 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/47033 |
en |
dc.description.abstract |
OBJECTIVE:Bacterial canker is a destructive disease of kiwifruit caused by the Gram-negative bacterium Pseudomonas syringae pv. actinidiae (Psa). To understand the disease-causing mechanism of Psa, a kiwifruit yeast two-hybrid cDNA library was constructed to identify putative host targets of the Psa Type Three Secreted Effector AvrPto5. RESULTS:In this study, we used the Mate & Plate™ yeast two-hybrid library method for constructing a kiwifruit cDNA library from messenger RNA of young leaves. The constructed library consisted of 2.15 × 106 independent clones with an average insert size of 1.52 kb. The screening of the kiwifruit yeast two-hybrid cDNA library with Psa AvrPto5 revealed the interaction of a V-type proton ATPase subunit-H, a proline rich-protein and heavy metal-associated isoprenylated plant protein 26. Among these, heavy metal-associated isoprenylated plant protein 26 showed a positive interaction with Psa AvrPto5 as both prey and bait. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
BMC research notes |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Pseudomonas syringae |
en |
dc.subject |
Yeasts |
en |
dc.subject |
Actinidia |
en |
dc.subject |
Fruit |
en |
dc.subject |
Plant Leaves |
en |
dc.subject |
Bacterial Proteins |
en |
dc.subject |
Plant Diseases |
en |
dc.subject |
Gene Library |
en |
dc.title |
Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1186/s13104-019-4102-x |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
63 |
en |
pubs.volume |
12 |
en |
dc.rights.holder |
Copyright: The authors |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
brief-report |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
761491 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
dc.identifier.eissn |
1756-0500 |
en |
pubs.record-created-at-source-date |
2019-01-30 |
en |
pubs.dimensions-id |
30691538 |
en |