Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5.

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dc.contributor.author Dharmaraj, Karthikeyan en
dc.contributor.author Cui, Wei en
dc.contributor.author Rikkerink, Erik HA en
dc.contributor.author Templeton, Matthew en
dc.date.accessioned 2019-06-14T01:57:48Z en
dc.date.issued 2019-01-28 en
dc.identifier.citation BMC research notes 12(1):63 28 Jan 2019 en
dc.identifier.issn 1756-0500 en
dc.identifier.uri http://hdl.handle.net/2292/47033 en
dc.description.abstract OBJECTIVE:Bacterial canker is a destructive disease of kiwifruit caused by the Gram-negative bacterium Pseudomonas syringae pv. actinidiae (Psa). To understand the disease-causing mechanism of Psa, a kiwifruit yeast two-hybrid cDNA library was constructed to identify putative host targets of the Psa Type Three Secreted Effector AvrPto5. RESULTS:In this study, we used the Mate & Plate™ yeast two-hybrid library method for constructing a kiwifruit cDNA library from messenger RNA of young leaves. The constructed library consisted of 2.15 × 106 independent clones with an average insert size of 1.52 kb. The screening of the kiwifruit yeast two-hybrid cDNA library with Psa AvrPto5 revealed the interaction of a V-type proton ATPase subunit-H, a proline rich-protein and heavy metal-associated isoprenylated plant protein 26. Among these, heavy metal-associated isoprenylated plant protein 26 showed a positive interaction with Psa AvrPto5 as both prey and bait. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries BMC research notes en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Pseudomonas syringae en
dc.subject Yeasts en
dc.subject Actinidia en
dc.subject Fruit en
dc.subject Plant Leaves en
dc.subject Bacterial Proteins en
dc.subject Plant Diseases en
dc.subject Gene Library en
dc.title Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5. en
dc.type Journal Article en
dc.identifier.doi 10.1186/s13104-019-4102-x en
pubs.issue 1 en
pubs.begin-page 63 en
pubs.volume 12 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype brief-report en
pubs.subtype Journal Article en
pubs.elements-id 761491 en
pubs.org-id Science en
pubs.org-id Biological Sciences en
dc.identifier.eissn 1756-0500 en
pubs.record-created-at-source-date 2019-01-30 en
pubs.dimensions-id 30691538 en


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https://creativecommons.org/licenses/by/4.0/ Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/

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