Nasal microbial composition and chronic otitis media with effusion: A case-control study.

Show simple item record Walker, Rebecca en Walker, Caroline en Camargo, Carlos en Bartley, Jim en Flint, David en Thompson, John en Mitchell, Edwin en 2019-06-14T02:33:58Z en 2019-01 en
dc.identifier.citation PloS One 14(2):e0212473 Jan 2019 en
dc.identifier.issn 1932-6203 en
dc.identifier.uri en
dc.description.abstract OBJECTIVES:Chronic otitis media with effusion (COME) in children can cause prolonged hearing loss, which is associated with an increased risk of learning delays and behavioural problems. Dispersal of bacterial pathogens from the nasal passages to the middle ear is implicated in COME. We sought to determine whether there is an association between nasal microbial composition and COME in children. METHODS:A case-control study of children aged 3 and 4 years was conducted. Cases undergoing placement of tympanostomy tubes for COME were compared to healthy controls. Nasal swabs were collected and a questionnaire was administered. The V1-3 region of the 16S rRNA gene was amplified, and sequenced on the Illumina MiSeq. RESULTS:73 children with COME had a lower Shannon diversity index than 105 healthy controls (1.62 [.80] versus 1.88 [.84], respectively; P = .046). The nasal microbiota of cases and controls differed in composition using Bray-Curtis dissimilarity (p = 0.002). Children with COME had a higher abundance of otopathogens and lower abundance of commensals including alpha haemolytic Streptococci and Lactococcus. Cluster analysis revealed 4 distinct nasal microbial profiles. Profiles that were Corynebacterium-dominated (aOR 4.18 [95%CI, 1.68-10.39], Streptococcus-dominated (aOR 3.12 [95%CI, 1.08-9.06], or Moraxella-dominated (aOR 4.70 [95%CI, 1.73-12.80] were associated with COME, compared to a more mixed microbial profile when controlling for age, ethnicity, and recent antibiotics use. CONCLUSIONS:Children with COME have a less diverse nasal microbial composition with a higher abundance of pathogens, compared to healthy children who have a more mixed bacterial profile with a higher abundance of commensals. Further research is required to determine how nasal microbiota may relate to the pathogenesis or maintenance of COME, and whether modification of the nasal microbiota can prevent or treat children at risk of COME. en
dc.format.medium Electronic-eCollection en
dc.language eng en
dc.relation.ispartofseries PloS one en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.subject Nasal Mucosa en
dc.subject Ear, Middle en
dc.subject Humans en
dc.subject Otitis Media with Effusion en
dc.subject Chronic Disease en
dc.subject DNA, Bacterial en
dc.subject Middle Ear Ventilation en
dc.subject Case-Control Studies en
dc.subject Biodiversity en
dc.subject Symbiosis en
dc.subject Species Specificity en
dc.subject Child, Preschool en
dc.subject Female en
dc.subject Male en
dc.subject Microbiota en
dc.title Nasal microbial composition and chronic otitis media with effusion: A case-control study. en
dc.type Journal Article en
dc.identifier.doi 10.1371/journal.pone.0212473 en
pubs.issue 2 en
pubs.begin-page e0212473 en
pubs.volume 14 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 765630 en Medical and Health Sciences en Population Health en Social & Community Health en School of Medicine en Paediatrics Child & Youth Hlth en
dc.identifier.eissn 1932-6203 en
pubs.record-created-at-source-date 2019-02-23 en
pubs.dimensions-id 30794625 en

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