Genomics of Hybridisation in New Zealand Stick Insects

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dc.contributor.advisor Buckley, T en McKey, Heather en 2019-08-07T03:26:36Z en 2019 en
dc.identifier.uri en
dc.description Full Text is available to authenticated members of The University of Auckland only. en
dc.description.abstract There are three species in the stick insect genus Clitarchus; Clitatarchus hookeri, Clitarchus tepaki and Clitarchus rakuwhakanekeneke. It has been previously suggested that C. tepaki was isolated on two Pliocene islands in Northland and subsequently formed two independent hybrid zones with C. hookeri following the formation of Karikari and Aupouri peninsulas. It was proposed that a stable hybrid swarm formed on the Aupouri peninsula and genetic swamping occurred on the Karikari peninsula. In these hybrid zones the patterns of divergence and amounts of introgression are unknown. Ecological and evolutionary interactions between lineages which have experienced divergence are able to be analysed using hybrid zones. Investigation of hybridisation was done by analysing population structure, speciation models and patterns of introgression, a large number of single nucleotide polymorphisms (SNPs) were obtained using Genotyping-by-sequencing (GBS) on 273 individuals sampled from 14 populations across the Karikari and Aupouri peninsulas. Before downstream analyses could be done, sequence alignment, genotyping and SNP calling were done using BWA and Stacks to create a SNP dataset required as input. Using population structure analysis, coancestry matrices, and analysing patterns of introgression there is evidence to support the hypothesis that along the Aupouri peninsula there is a stable hybrid zone however on the Karikari peninsula genetic swamping has occurred masking any C. tepaki signal. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.relation.isreferencedby UoA99265168913602091 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Full Text is available to authenticated members of The University of Auckland only. en
dc.rights.uri en
dc.rights.uri en
dc.title Genomics of Hybridisation in New Zealand Stick Insects en
dc.type Thesis en Biological Sciences en The University of Auckland en Masters en
dc.rights.holder Copyright: The author en
pubs.elements-id 778400 en
pubs.record-created-at-source-date 2019-08-07 en

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