dc.contributor.author |
Walter, U |
en |
dc.contributor.author |
Miller, P |
en |
dc.contributor.author |
Wilson, F |
en |
dc.contributor.author |
Menkes, David |
en |
dc.contributor.author |
Greengard, P |
en |
dc.date.accessioned |
2019-09-19T03:00:37Z |
en |
dc.date.issued |
1980 |
en |
dc.identifier.citation |
Journal of Biological Chemistry 255(8):3757-3762 1980 |
en |
dc.identifier.issn |
0021-9258 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/47786 |
en |
dc.description.abstract |
A guanosine 3':5'-monophosphate (cGMP)-dependent protein kinase was purified from bovine lung using 8-(6-aminohexylamino)-cAMP-Sepharose. The activity of the purified enzyme was highly dependent on cGMP using histone f2b as a substrate. The self-phosphorylation of the purified enzyme was strongly inhibited by cGMP and not significantly affected by cAMP. A precipitating antiserum prepared in rabbits against the cGMP-dependent protein kinase specifically inhibited the histone kinase activity and the self-phosphorylation of the purified cGMP-dependent protein kinase without affecting the cGMP binding site. This antiserum also specifically inhibited the phosphorylation of the endogenous substrate proteins by endogenous cGMP-dependent protein kinase in smooth muscle membranes, but did not cross-react detectably with catalytic subunit or regulatory subunit of type I or type II cAMP-dependent protein kinase. Conversely, anti-sera against the regulatory subunit of type I or type II cAMP-dependent protein kinase did not cross-react detectably with cGMP-dependent protein kinase. The substantial differences between the immunological properties of the cGMP-dependent and cAMP-dependent protein kinases suggest that these two enzymes have distinct physiological roles. |
en |
dc.publisher |
American Society for Biochemistry and Molecular Biology, Inc. |
en |
dc.relation.ispartofseries |
Journal of Biological Chemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Immunological distinction between guanosine 3': 5' - monophosphate-dependent and adenosine 3': 5' - monophosphate-dependent protein kinases |
en |
dc.type |
Journal Article |
en |
pubs.issue |
8 |
en |
pubs.begin-page |
3757 |
en |
pubs.volume |
255 |
en |
dc.rights.holder |
Copyright: American Society for Biochemistry and Molecular Biology |
en |
pubs.author-url |
http://www.jbc.org/content/255/8/3757.long |
en |
pubs.end-page |
3762 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
774617 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Psychological Medicine Dept |
en |
pubs.record-created-at-source-date |
2019-06-17 |
en |
pubs.dimensions-id |
6154053 |
en |