Immunological distinction between guanosine 3': 5' - monophosphate-dependent and adenosine 3': 5' - monophosphate-dependent protein kinases

ResearchSpace/Manakin Repository

Show simple item record

dc.contributor.author Walter, U en
dc.contributor.author Miller, P en
dc.contributor.author Wilson, F en
dc.contributor.author Menkes, David en
dc.contributor.author Greengard, P en
dc.date.accessioned 2019-09-19T03:00:37Z en
dc.date.issued 1980 en
dc.identifier.citation Journal of Biological Chemistry 255(8):3757-3762 1980 en
dc.identifier.issn 0021-9258 en
dc.identifier.uri http://hdl.handle.net/2292/47786 en
dc.description.abstract A guanosine 3':5'-monophosphate (cGMP)-dependent protein kinase was purified from bovine lung using 8-(6-aminohexylamino)-cAMP-Sepharose. The activity of the purified enzyme was highly dependent on cGMP using histone f2b as a substrate. The self-phosphorylation of the purified enzyme was strongly inhibited by cGMP and not significantly affected by cAMP. A precipitating antiserum prepared in rabbits against the cGMP-dependent protein kinase specifically inhibited the histone kinase activity and the self-phosphorylation of the purified cGMP-dependent protein kinase without affecting the cGMP binding site. This antiserum also specifically inhibited the phosphorylation of the endogenous substrate proteins by endogenous cGMP-dependent protein kinase in smooth muscle membranes, but did not cross-react detectably with catalytic subunit or regulatory subunit of type I or type II cAMP-dependent protein kinase. Conversely, anti-sera against the regulatory subunit of type I or type II cAMP-dependent protein kinase did not cross-react detectably with cGMP-dependent protein kinase. The substantial differences between the immunological properties of the cGMP-dependent and cAMP-dependent protein kinases suggest that these two enzymes have distinct physiological roles. en
dc.publisher American Society for Biochemistry and Molecular Biology, Inc. en
dc.relation.ispartofseries Journal of Biological Chemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Immunological distinction between guanosine 3': 5' - monophosphate-dependent and adenosine 3': 5' - monophosphate-dependent protein kinases en
dc.type Journal Article en
pubs.issue 8 en
pubs.begin-page 3757 en
pubs.volume 255 en
dc.rights.holder Copyright: American Society for Biochemistry and Molecular Biology en
pubs.author-url http://www.jbc.org/content/255/8/3757.long en
pubs.end-page 3762 en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Article en
pubs.elements-id 774617 en
pubs.org-id Medical and Health Sciences en
pubs.org-id School of Medicine en
pubs.org-id Psychological Medicine Dept en
pubs.record-created-at-source-date 2019-06-17 en
pubs.dimensions-id 6154053 en


Full text options

Find Full text

This item appears in the following Collection(s)

Show simple item record

Share

Search ResearchSpace


Advanced Search

Browse