A versatile cancer cell trapping and 1D migration assay in a microfluidic device

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dc.contributor.author Hisey, Colin en
dc.contributor.author Mitxelena-Iribarren, O en
dc.contributor.author Martinez-Calderon, M en
dc.contributor.author Gordon, JB en
dc.contributor.author Olaizola, SM en
dc.contributor.author Benavente-Babace, A en
dc.contributor.author Mujika, M en
dc.contributor.author Arana, S en
dc.contributor.author Hansford, DJ en
dc.date.accessioned 2019-09-19T03:54:44Z en
dc.date.issued 2019-07-23 en
dc.identifier.citation Biomicrofluidics 13(4):044105-1-044105-8 23 Jul 2019 en
dc.identifier.issn 1932-1058 en
dc.identifier.uri http://hdl.handle.net/2292/47821 en
dc.description.abstract Highly migratory cancer cells often lead to metastasis and recurrence and are responsible for the high mortality rates in many cancers despite aggressive treatment. Recently, the migratory behavior of patient-derived glioblastoma multiforme cells on microtracks has shown potential in predicting the likelihood of recurrence, while at the same time, antimetastasis drugs have been developed which require simple yet relevant high-throughput screening systems. However, robust in vitro platforms which can reliably seed single cells and measure their migration while mimicking the physiological tumor microenvironment have not been demonstrated. In this study, we demonstrate a microfluidic device which hydrodynamically seeds single cancer cells onto stamped or femtosecond laser ablated polystyrene microtracks, promoting 1D migratory behavior due to the cells’ tendency to follow topographical cues. Using time-lapse microscopy, we found that single U87 glioblastoma multiforme cells migrated more slowly on laser ablated microtracks compared to stamped microtracks of equal width and spacing (p < 0.05) and exhibited greater directional persistence on both 1D patterns compared to flat polystyrene (p < 0.05). Single-cell morphologies also differed significantly between flat and 1D patterns, with cells on 1D substrates exhibiting higher aspect ratios and less circularity (p < 0.05). This microfluidic platform could lead to automated quantification of single-cell migratory behavior due to the high predictability of hydrodynamic seeding and guided 1D migration, an important step to realizing the potential of microfluidic migration assays for drug screening and individualized medicine. en
dc.publisher AIP Publishing en
dc.relation.ispartofseries Biomicrofluidics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://publishing.aip.org/resources/researchers/rights-and-permissions/sharing-content-online/ en
dc.title A versatile cancer cell trapping and 1D migration assay in a microfluidic device en
dc.type Journal Article en
dc.identifier.doi 10.1063/1.5103269 en
pubs.issue 4 en
pubs.begin-page 044105-1 en
pubs.volume 13 en
dc.rights.holder Copyright: The author(s) en
pubs.end-page 044105-8 en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Article en
pubs.elements-id 777342 en
pubs.org-id Medical and Health Sciences en
pubs.org-id School of Medicine en
pubs.org-id Obstetrics and Gynaecology en
pubs.record-created-at-source-date 2019-07-28 en
pubs.online-publication-date 2019-07-23 en


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