Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids.

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dc.contributor.author Hari, Neelam en
dc.contributor.author Patel, Priyanka en
dc.contributor.author Ross, Jacqueline en
dc.contributor.author Hicks, Kevin en
dc.contributor.author Vanholsbeeck, Frederique en
dc.date.accessioned 2019-09-23T22:23:13Z en
dc.date.issued 2019-07-22 en
dc.identifier.citation Scientific reports 9(1):10601 22 Jul 2019 en
dc.identifier.issn 2045-2322 en
dc.identifier.uri http://hdl.handle.net/2292/47915 en
dc.description.abstract Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous cells to drugs, optimised imaging protocols are required. This study uses a simple custom-built spectral domain optical coherence tomography (OCT) system to conduct RI measurements of multicellular spheroids, three-dimensional (3D) in-vitro culture systems, of the cell line HCT116. The spheroid RIs are compared to study the effect of growth over time. To improve confocal microscopy imaging protocols, two immersion media (glycerol and ScaleView-A2) matching the spheroid RIs were trialled, with the aim to reduce the RI mismatch between the spheroid and the immersion medium and thus improve imaging depth with confocal microscopy. ScaleView-A2 (n = 1.380) aided in achieving greater depths of imaging of the multicellular spheroids under confocal microscopy. This improvement in imaging depth confirmed the utility of our RI measurements, proving the promising outlook of OCT as a complementary tool to microscopy in cancer research. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Scientific reports en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.title Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids. en
dc.type Journal Article en
dc.identifier.doi 10.1038/s41598-019-47000-2 en
pubs.issue 1 en
pubs.begin-page 10601 en
pubs.volume 9 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 778505 en
pubs.org-id Science en
pubs.org-id Physics en
dc.identifier.eissn 2045-2322 en
pubs.record-created-at-source-date 2019-07-24 en
pubs.dimensions-id 31332221 en

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https://creativecommons.org/licenses/by/4.0/ Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/


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