An ultra-fast mechanically active cell culture substrate.

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Show simple item record Poulin, Alexandre en Imboden, Matthias en Sorba, Francesca en Grazioli, Serge en Martin-Olmos, Cristina en Rosset, Samuel en Shea, Herbert en 2019-09-23T22:23:19Z en 2018-07-02 en
dc.identifier.citation Scientific reports 8(1):9895 02 Jul 2018 en
dc.identifier.issn 2045-2322 en
dc.identifier.uri en
dc.description.abstract We present a mechanically active cell culture substrate that produces complex strain patterns and generates extremely high strain rates. The transparent miniaturized cell stretcher is compatible with live cell microscopy and provides a very compact and portable alternative to other systems. A cell monolayer is cultured on a dielectric elastomer actuator (DEA) made of a 30 μm thick silicone membrane sandwiched between stretchable electrodes. A potential difference of several kV's is applied across the electrodes to generate electrostatic forces and induce mechanical deformation of the silicone membrane. The DEA cell stretcher we present here applies up to 38% tensile and 12% compressive strain, while allowing real-time live cell imaging. It reaches the set strain in well under 1 ms and generates strain rates as high as 870 s-1, or 87%/ms. With the unique capability to stretch and compress cells, our ultra-fast device can reproduce the rich mechanical environment experienced by cells in normal physiological conditions, as well as in extreme conditions such as blunt force trauma. This new tool will help solving lingering questions in the field of mechanobiology, including the strain-rate dependence of axonal injury and the role of mechanics in actin stress fiber kinetics. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Scientific reports en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.subject Stress Fibers en
dc.subject Humans en
dc.subject Elastomers en
dc.subject Fluorescent Dyes en
dc.subject Microscopy, Fluorescence en
dc.subject Cell Culture Techniques en
dc.subject Equipment Design en
dc.subject Electrodes en
dc.subject Stress, Mechanical en
dc.subject Tensile Strength en
dc.subject A549 Cells en
dc.title An ultra-fast mechanically active cell culture substrate. en
dc.type Journal Article en
dc.identifier.doi 10.1038/s41598-018-27915-y en
pubs.issue 1 en
pubs.begin-page 9895 en
pubs.volume 8 en
dc.rights.holder Copyright: The author en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 747688 en Bioengineering Institute en
dc.identifier.eissn 2045-2322 en
pubs.record-created-at-source-date 2018-07-04 en
pubs.dimensions-id 29967520 en

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