dc.contributor.author |
Correddu, Danilo |
en |
dc.contributor.author |
Montaño López, José de Jesús |
en |
dc.contributor.author |
Vadakkedath, Praveen George |
en |
dc.contributor.author |
Lai, Amy |
en |
dc.contributor.author |
Pernes, Jane I |
en |
dc.contributor.author |
Watson, Paris R |
en |
dc.contributor.author |
Leung, Ka Ho Ivanhoe |
en |
dc.date.accessioned |
2019-09-23T23:03:40Z |
en |
dc.date.issued |
2019-06-20 |
en |
dc.identifier.citation |
Scientific reports 9(1):8884 20 Jun 2019 |
en |
dc.identifier.issn |
2045-2322 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/47925 |
en |
dc.description.abstract |
Human ribosomal proteins play important structural and functional roles in the ribosome and in protein synthesis. An efficient method to recombinantly produce and purify these proteins would enable their full characterisation. However, the production of human ribosomal proteins can be challenging. The only published method about the recombinant production of human ribosomal proteins involved the recovery of proteins from inclusion bodies, a process that is tedious and may lead to significant loss of yield. Herein, we explored the use of different Escherichia coli competent cells and fusion protein tags for the recombinant production of human ribosomal proteins. We found that, by using thioredoxin as a fusion protein, soluble ribosomal protein could be obtained directly from cell lysates, thus leading to an improved method to recombinantly produce these proteins. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Scientific reports |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.title |
An improved method for the heterologous production of soluble human ribosomal proteins in Escherichia coli. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1038/s41598-019-45323-8 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
8884 |
en |
pubs.volume |
9 |
en |
dc.rights.holder |
Copyright: The authors |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
775456 |
en |
pubs.org-id |
Academic Services |
en |
pubs.org-id |
Examinations |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Chemistry |
en |
dc.identifier.eissn |
2045-2322 |
en |
pubs.record-created-at-source-date |
2019-06-22 |
en |
pubs.dimensions-id |
31222068 |
en |